Liu Bing, Wang Hai-Feng, Zhang Li-Hua, Liu Fang, He Feng-Jun, Bai Jiao, Hua Hui-Ming, Chen Gang, Pei Yue-Hu
a School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University , Shenyang 110016 , P.R. China.
b Key Laboratory of Structure-Based Drug Design and Discovery (Shenyang Pharmaceutical University), Ministry of Education , Shenyang 110016 , P.R. China.
Nat Prod Res. 2015;29(23):2197-202. doi: 10.1080/14786419.2015.1008472. Epub 2015 May 13.
Strain HSY05 was isolated from sea sediment collected from the South China Sea and was later identified as Penicillium oxalicum by 16S rDNA sequence analysis. Various chromatographic processes led to the isolation and purification of two metabolites from the fermentation culture of HSY05, including one new compound, 2,2',4,4'-tetrahyoxy-8'-methyl-6-methoxy-acyl-ethyl-diphenylmethanone (1), and a known compound secalonic acid D (SAD, 2), as characterised by UV, IR, 1D, 2D-NMR and MS data. The inhibitory activities against topoisomerase I of these two compounds were evaluated. The result showed that in addition to the known topo I inhibitor SAD (2), compound 1 also exhibited a moderate inhibitory effect.
菌株HSY05是从中国南海采集的海洋沉积物中分离得到的，随后通过16S rDNA序列分析鉴定为草酸青霉。通过各种色谱方法从HSY05的发酵培养物中分离并纯化出两种代谢产物，其中包括一种新化合物2,2',4,4'-四羟基-8'-甲基-6-甲氧基-酰基-乙基-二苯基甲酮（1），以及一种已知化合物secalonic酸D（SAD，2），通过紫外、红外、一维、二维核磁共振和质谱数据对其进行了表征。评估了这两种化合物对拓扑异构酶I的抑制活性。结果表明，除了已知的拓扑异构酶I抑制剂SAD（2）外，化合物1也表现出中等程度的抑制作用。
