Department of Natural Sciences, School of Arts and Sciences, Lebanese American University, PO Box 36, Byblos, Lebanon.
Inflamm Res. 2015 Jul;64(7):501-12. doi: 10.1007/s00011-015-0831-z. Epub 2015 May 13.
This study is aimed at evaluating the effects of a cafeteria diet (obesity) mouse model on early multi-organ functional, structural, endocrine and biochemical alterations.
Multi-organ damage is assessed using clinical, biochemical, pathological, and inflammatory parameters in 30 mice fed one of the three diets for 15 weeks: standard chow diet (SC), high fat (HF), or "Cafeteria diet" (CAF) (standard SC and a choice of highly palatable human cafeteria foods: chocolate, biscuits, and peanut butter).
CAF diet was associated with an increase in body weight, energy intake, and serum cholesterol levels compared to the other diets, as well as higher insulin levels and lower glucose tolerance. Additionally, consumption of the CAF diet was associated with significantly higher weight gain, abdominal fat, and serum IL-6 levels, as well as more damage in the heart (coronary perivascular fibrosis and steatosis), kidney (chronic interstitial inflammation and glomerular sclerosis), and liver (liver weight, portal fibrosis, apoptosis, and steatosis) compared to the HF diet.
Functional and structural damage in CAF were higher than HF of similar macronutrient composition. This study provides a novel dietary model in mice that mimics multi-organ physiologic alterations in humans secondary to obesity.
本研究旨在评估自助餐饮食（肥胖）小鼠模型对早期多器官功能、结构、内分泌和生化改变的影响。
通过临床、生化、病理和炎症参数评估 30 只小鼠的多器官损伤，这些小鼠在 15 周内分别喂食三种饮食中的一种：标准饲料饮食（SC）、高脂肪饮食（HF）或“自助餐饮食”（CAF）（标准 SC 和多种美味的人类自助餐食物选择：巧克力、饼干和花生酱）。
与其他饮食相比，CAF 饮食与体重增加、能量摄入和血清胆固醇水平升高有关，同时胰岛素水平升高，葡萄糖耐量降低。此外，与 HF 饮食相比，CAF 饮食还与更高的体重增加、腹部脂肪和血清 IL-6 水平、心脏（冠状动脉血管周围纤维化和脂肪变性）、肾脏（慢性间质炎症和肾小球硬化）和肝脏（肝重、门脉纤维化、细胞凋亡和脂肪变性）的损伤显著增加有关。
CAF 的功能和结构损伤高于 HF，但两者的宏量营养素组成相似。本研究在小鼠中提供了一种新的饮食模型，模拟了肥胖导致的人类多器官生理改变。
