Al-Dalaeen Anfal, Batarseh Nour, Abdelhadi Nadine N, Atawneh Sally, AbuKashef Reem, Al-Yasari Ali Mosa Ra-Shid
Department of Clinical Nutrition and Dietetics, Faculty of Allied Medical Sciences, Applied Science Private University, Amman 11937, Jordan.
Department of Clinical Nutrition and Dietetics, Faculty of Allied Medical Sciences, Philadelphia University, Amman 19392, Jordan.
Medicina (Kaunas). 2025 Feb 4;61(2):263. doi: 10.3390/medicina61020263.
: Obesity has increased worldwide, and existing anti-obesity medications have treatment limitations that diminish their overall benefits. This study aimed to investigate the effects of orlistat in combination with "Cardamom" (CAR) extract on working memory, recognition memory, anxiety, and inflammation within hippocampal tissue. : Mice were categorized into two groups: a control group (CD) and a cafeteria diet (CAF) group induced with obesity (CAF) for 10 weeks. The groups were then subdivided into a CAF group treated with orlistat (CAF-ORL), a CAF group treated with orlistat and (CAF-ORL-CARD), and a group that continued on the CAF. The CAF-ORL group received orlistat at a dosage of 10 mg/kg/day for four weeks, while the CAF-ORL-CARD group received 10 mg/kg/day of orlistat and 500 mg/kg of CAR extract via oral gavage. In the 14th week, various assessments were conducted, including the novel object recognition (NOR) test, Y maze test, marble-burying test (MBT), open-field test, and TNF-α levels in the hippocampus. : TNF-α levels in the hippocampal tissue of the CAF group were elevated compared to the CD group ( < 0.01), whereas the CAF-ORL group exhibited reduced TNF-α levels compared to the CAF group ( < 0.01). Moreover, TNF-α levels in the CAF-ORL-CARD group were significantly lower than in the CAF-ORL group ( < 0.01). The recognition index was notably higher in the CAF-ORL group compared to the CAF group ( < 0.01) and higher in the CAF-ORL-CARD group compared to the CAF-ORL group ( < 0.01). However, there were no changes in the open-field test and Y maze test ( > 0.05). : Orlistat combined with CAR has positive effects on neuroinflammation and memory, suggesting that this combination may offer potential therapeutic benefits for cognitive impairments and hippocampal dysfunction associated with obesity.
肥胖在全球范围内呈上升趋势，现有的抗肥胖药物存在治疗局限性，降低了它们的整体益处。本研究旨在探讨奥利司他与小豆蔻（CAR）提取物联合使用对海马组织内工作记忆、识别记忆、焦虑和炎症的影响。
对照组（CD）和通过喂食自助餐饮食诱导肥胖10周的自助餐饮食（CAF）组。然后将这些组再细分为接受奥利司他治疗的CAF组（CAF-ORL）、接受奥利司他和CAR提取物治疗的CAF组（CAF-ORL-CARD）以及继续采用自助餐饮食的组。CAF-ORL组连续四周每天以10mg/kg的剂量接受奥利司他治疗，而CAF-ORL-CARD组通过灌胃接受10mg/kg/天的奥利司他和500mg/kg的CAR提取物。在第14周，进行了各种评估，包括新物体识别（NOR）测试、Y迷宫测试、埋大理石试验（MBT）、旷场试验以及海马中的肿瘤坏死因子-α（TNF-α）水平检测。
与CD组相比，CAF组海马组织中的TNF-α水平升高（P<0.01），而与CAF组相比，CAF-ORL组的TNF-α水平降低（P<0.01）。此外，CAF-ORL-CARD组中的TNF-α水平显著低于CAF-ORL组（P<0.01）。与CAF组相比，CAF-ORL组的识别指数显著更高（P<0.01），与CAF-ORL组相比，CAF-ORL-CARD组的识别指数更高（P<0.01）。然而，旷场试验和Y迷宫测试中没有变化（P>0.05）。
奥利司他与CAR联合使用对神经炎症和记忆有积极影响，表明这种联合可能为与肥胖相关的认知障碍和海马功能障碍提供潜在的治疗益处。
