Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LABMET), Ghent University, Coupure Links 653, 9000, Ghent, Belgium.
Appl Microbiol Biotechnol. 2015 Sep;99(17):7307-20. doi: 10.1007/s00253-015-6646-6. Epub 2015 May 13.
The methane removal capacity of mixed methane-oxidizing bacteria (MOB) culture in a biofilter setup using autoclaved aerated concrete (AAC) as a highly porous carrier material was tested. Batch experiment was performed to optimize MOB immobilization on AAC specimens where optimum methane removal was obtained when calcium chloride was not added during bacterial inoculation step and 10-mm-thick AAC specimens were used. The immobilized MOB could remove methane at low concentration (~1000 ppmv) in a biofilter setup for 127 days at average removal efficiency (RE) of 28.7 %. Unlike a plug flow reactor, increasing the total volume of the filter by adding a biofilter in series did not result in higher total RE. MOB also exhibited a higher abundance at the bottom of the filter, in proximity with the methane gas inlet where a high methane concentration was found. Overall, an efficient methane biofilter performance could be obtained using AAC as the carrier material.
采用高压蒸汽灭菌加气混凝土（AAC）作为高多孔载体材料的生物滤池装置中混合甲烷氧化菌（MOB）的甲烷去除能力进行了测试。进行了批量实验以优化 MOB 在 AAC 标本上的固定化，在细菌接种步骤中不添加氯化钙且使用 10 毫米厚的 AAC 标本时，可获得最佳的甲烷去除效果。固定化的 MOB 可以在生物滤池装置中去除低浓度（约 1000ppmv）的甲烷，在 127 天的平均去除效率（RE）为 28.7%。与推流式反应器不同，通过串联添加生物滤池来增加过滤器的总体积并不会导致总 RE 更高。MOB 在靠近甲烷气体入口的过滤器底部也有更高的丰度，在那里发现了高浓度的甲烷。总体而言，使用 AAC 作为载体材料可以获得高效的甲烷生物滤池性能。
