Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China.
Bioresour Technol. 2018 May;256:201-207. doi: 10.1016/j.biortech.2018.02.020. Epub 2018 Feb 7.
Methane in exhaust gas from biogas upgrading process, which is a greenhouse gas, could cause global warming. The biofilter with immobilized methane-oxidizing bacteria (MOB) is a promising approach for methane removal, and the selections of inoculated MOB culture and support material are vital for the biofilter. In this work, five MOB consortia were enriched at different methane concentrations. The MOB-20 consortium enriched at the methane concentration of 20.0% (v/v) was then immobilized on sponge and two particle sizes of volcanic rock in biofilters to remove methane in exhaust gas from biogas upgrading process. Results showed that the immobilized MOB performed more admirable methane removal capacity than suspended cells. The immobilized MOB on sponge reached the highest methane removal efficiency (RE) of 35%. The rough surface, preferable hydroscopicity, appropriate pore size and particle size of support material might favor the MOB immobilization and accordingly methane removal.
沼气升级过程中废气中的甲烷是一种温室气体，可能会导致全球变暖。固定化甲烷氧化菌（MOB）的生物过滤器是一种很有前途的去除甲烷的方法，接种 MOB 培养物和支撑材料的选择对于生物过滤器至关重要。在这项工作中，在不同的甲烷浓度下富集了五个 MOB 群落。然后，将在 20.0%（v/v）甲烷浓度下富集的 MOB-20 群落固定在海绵和两种粒径的火山岩上，以去除沼气升级过程中废气中的甲烷。结果表明，固定化 MOB 比悬浮细胞具有更令人满意的甲烷去除能力。固定在海绵上的 MOB 达到了 35%的最高甲烷去除效率（RE）。支撑材料的粗糙表面、良好的吸水性、适当的孔径和粒径可能有利于 MOB 的固定化，从而有利于甲烷的去除。
