银涂层对大肠杆菌黏附于导尿管影响的体外评估
In vitro evaluation of the impact of silver coating on Escherichia coli adherence to urinary catheters.
作者信息
Ogilvie Adam T, Brisson Brigitte A, Singh Ameet, Weese J Scott
机构信息
Departments of Clinical Studies (Ogilvie, Brisson, Singh) and Department of Pathobiology (Weese), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
出版信息
Can Vet J. 2015 May;56(5):490-4.
A silver-coated urinary catheter was compared to a non-silver-coated urinary catheter for the ability to reduce adherence of 6 isolates of Escherichia coli. Catheters were incubated with E. coli strains for 0, 24, 48, and 72 h. Broth was sampled at all time points to determine CFU/mL. Catheters were subjected to sonication to determine adhered bacteria at all time points, and scanning electron microscopy (SEM) to semi-quantitatively assess biofilm formation. Silver-coated catheters had significantly less adhered bacteria than non-silver-coated catheters at times 24, 48, and 72 h. Subjectively, silver-coated urinary catheters had less biofilm formation than non-silver-coated urinary catheters as assessed by SEM. Silver coating of catheters was associated with reduced adherence of E. coli in an in vitro evaluation. Testing of catheters in dogs in vivo is required to determine if there is a reduction in catheter-associated urinary tract infections.
将涂银导尿管与未涂银导尿管在减少6株大肠杆菌黏附能力方面进行了比较。将导尿管与大肠杆菌菌株一起孵育0、24、48和72小时。在所有时间点采集肉汤样本以测定每毫升菌落形成单位（CFU/mL）。对导尿管进行超声处理以确定所有时间点的黏附细菌，并通过扫描电子显微镜（SEM）半定量评估生物膜形成。在24、48和72小时时，涂银导尿管上黏附的细菌明显少于未涂银导尿管。主观上，通过SEM评估，涂银导尿管形成的生物膜比未涂银导尿管少。在体外评估中，导尿管的涂银与大肠杆菌黏附减少有关。需要在犬体内对导尿管进行测试，以确定是否能减少导尿管相关的尿路感染。
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