Verma Neelam, Singh Ashish Kumar
Department of Biotechnology, Punjabi University Patiala, Punjab, Patiala 147 002, India.
ISRN Biotechnol. 2012 Nov 27;2013:236062. doi: 10.5402/2013/236062. eCollection 2013.
A biosensor was developed for the determination of BOD value of fermentation industry effluent. The developed biosensor was fabricated by immobilizing the microbial consortium on cellulose acetate (CA) membrane in close proximity to a DO probe electrode. The microbial consortium was harvested from the fermentation industry effluent. The BOD biosensor was calibrated by using a solution containing the equivalent amount of glucose/glutamic acid (GGA) as a standard sample solution. The response time was optimized by immobilizing different concentrations of cell biomass on CA membrane. Once the response time was optimized, it was used for determination of BOD of fermentation industry effluent. For analysis of fermentation industry effluent, the response time was observed 7 minutes with detection limit 1 mg/L. Good linear range with GGA standard solution was observed, R (2) 0.99 with relative standard deviation (RSD) <%. The observed BOD value by biosensor showed a good comparison with the conventional method for the determination of BOD.
开发了一种用于测定发酵工业废水生化需氧量（BOD）值的生物传感器。所开发的生物传感器是通过将微生物群落固定在醋酸纤维素（CA）膜上，且靠近溶解氧（DO）探针电极来制备的。微生物群落取自发酵工业废水。通过使用含有等量葡萄糖/谷氨酸（GGA）的溶液作为标准样品溶液对BOD生物传感器进行校准。通过将不同浓度的细胞生物质固定在CA膜上来优化响应时间。一旦响应时间得到优化，就将其用于测定发酵工业废水的BOD。对于发酵工业废水的分析，观察到响应时间为7分钟，检测限为1mg/L。观察到与GGA标准溶液具有良好的线性范围，R（2）为0.99，相对标准偏差（RSD）<％。生物传感器测得的BOD值与测定BOD的传统方法具有良好的可比性。
