Bobryshev Iu V, Kuznetsov A S
Arkh Patol. 1989;51(9):20-6.
Structural characteristics of low density lipoprotein (LDL)-intimal cellular element interaction were studied in atherosclerotic lesions in the aortas obtained from rabbit with experimentally induced hypercholesterolemia and in human coronary arteries. An electron microscopic autoradiographic examination using 125I to study the in vivo interaction between LDL and rabbit aortic intimal cells indicated that the formation of foam cells was associated with activated endocyte activity and uncontrolled capture of atherogenic lipoproteins. The structural analysis of the interaction of native and modified LDL labelled with colloid gold, on the one hand, and human coronary intimal cells, on the other, was made by using ultrathin sections. This enabled the localization of the receptors to lipoproteins to be established. This also demonstrated that transformation of the cells to foam ones was due to the functional insufficiency of lysosomal activity and to the filling of the cytoplasm with membranelles and membrane-surrounded lipid vacuoles.
在实验性诱导高胆固醇血症的兔主动脉粥样硬化病变以及人类冠状动脉中，研究了低密度脂蛋白（LDL）与内膜细胞成分相互作用的结构特征。使用¹²⁵I进行电子显微镜放射自显影检查，以研究LDL与兔主动脉内膜细胞之间的体内相互作用，结果表明泡沫细胞的形成与内吞细胞活性激活以及致动脉粥样硬化脂蛋白的失控捕获有关。一方面，通过使用超薄切片对用胶体金标记的天然和修饰LDL与人类冠状动脉内膜细胞之间的相互作用进行结构分析。这使得能够确定脂蛋白受体的定位。这还表明细胞向泡沫细胞的转变是由于溶酶体活性的功能不足以及细胞质中充满了膜性小体和膜包绕的脂质空泡。
