Bhatt Sheelendra Mangal, Mohan Anand, Srivastava Suresh Kumar
Biotechnology Department, Lovely Professional University, Punjab 144 401, India.
Institute of Technology, Banaras Hindu University, Varanasi 221 005, India.
ISRN Biotechnol. 2012 Dec 26;2013:914187. doi: 10.5402/2013/914187. eCollection 2013.
Mannitol is an important biochemical often used as medicine and in food sector, yet its biotechnological is not preffered in Industry for large scale production, which may be due to the multistep mechanism involved in hydrogenation and reduction. This paper is a comparative preview covering present chemical and biotechnological approaches existing today for mannitol production at industrial scale. Biotechnological routes are suitable for adaptation at industrial level for mannitol production, and whatever concerns are there had been discussed in detail, namely, raw materials, broad range of enzymes with high activity at elevated temperature suitable for use in reactor, cofactor limitation, reduced by-product formation, end product inhibition, and reduced utilization of mannitol for enhancing the yield with maximum volumetric productivity.
甘露醇是一种重要的生物化学物质，常用于医药和食品领域，然而其生物技术法在工业大规模生产中并不受欢迎，这可能是由于氢化和还原过程涉及多步机制。本文是一篇比较综述，涵盖了目前工业规模生产甘露醇的化学和生物技术方法。生物技术路线适合在工业层面用于甘露醇生产，并且已详细讨论了存在的各种问题，即原材料、适用于反应器的在高温下具有高活性的多种酶、辅因子限制、减少副产物形成、终产物抑制以及减少甘露醇的利用以提高产量并实现最大体积生产率。
