Karunanithy Chinnadurai, Muthukumarappan Kasiviswanathan, Gibbons William R
Department of Agricultural Biosystems Engineering, South Dakota State University, 1400 North Campus Drive, Brookings, SD 57007, USA.
ISRN Biotechnol. 2012 May 29;2013:942810. doi: 10.5402/2013/942810. eCollection 2013.
Biofuels from biomass have the potential to reduce the dependency on fossil fuels. An efficient pretreatment method is required to accomplish the target of the Energy Act 2005. Extrusion could be a viable continuous pretreatment method to be explored. The objectives of the current study were to investigate the influence of screw speed and barrel temperature on sugar recovery from the selected warm season grasses and to select a suitable enzyme combination and dose for enzymatic hydrolysis. The ground, moisture-balanced biomasses were pretreated using a single screw extruder at various screw speeds (100, 150, and 200 rpm) and barrel temperatures (50, 75, 100, 150, and 200°C). Cellulase or multienzyme with β-glucosidase was varied from 1 : 1 to 1 : 4 during enzymatic hydrolysis to accomplish the second objective. Screw speed, barrel temperature, and their interaction had a significant influence on sugar recovery from the selected biomasses. A maximum of 28.2, 66.2, and 49.2% of combined sugar recoverywasachieved for switchgrass, big bluestem, prairie cord grass when pretreated at a screw speed of 200, 200, and 150 rpm and at a barrel temperature of 75, 150, and 100°C, respectively, using cellulase and β-glucosidase at a ratio of 1 : 4. Extrusion pretreatment of these biomasses used only 28-37% of the rated extruder power.
来自生物质的生物燃料有潜力减少对化石燃料的依赖。需要一种有效的预处理方法来实现2005年《能源法案》的目标。挤压可能是一种值得探索的可行的连续预处理方法。本研究的目的是研究螺杆转速和机筒温度对从选定的暖季草中回收糖分的影响，并选择合适的酶组合和剂量用于酶水解。将研磨并平衡水分的生物质在不同的螺杆转速（100、150和200转/分钟）和机筒温度（50、75、100、150和200°C）下使用单螺杆挤压机进行预处理。在酶水解过程中，纤维素酶或含β-葡萄糖苷酶的复合酶的比例从1∶1变化到1∶4，以实现第二个目标。螺杆转速、机筒温度及其相互作用对从选定生物质中回收糖分有显著影响。当分别以200、200和150转/分钟的螺杆转速以及75、150和100°C的机筒温度进行预处理，并使用比例为1∶4的纤维素酶和β-葡萄糖苷酶时，柳枝稷、大须芒草、草原绳草的总糖回收率分别最高可达28.2%、66.2%和49.2%。这些生物质的挤压预处理仅使用了挤出机额定功率的28 - 37%。
