Vijayaraghavan Ponnuswamy, Primiya R Raja, Prakash Vincent Samuel Gnana
Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari, Tamil Nadu 629 502, India.
P. G. Department of Microbiology, Ayya Nadar Janaki Ammal College, Sivakasi, Tamil Nadu 626 124, India.
ISRN Biotechnol. 2013 Dec 13;2013:394305. doi: 10.5402/2013/394305. eCollection 2013.
A bacterial isolate, Alcaligenes sp. secreting phytase (EC 3.1.3.8), was isolated and characterized. The optimum conditions for the production of phytase included a fermentation period of 96 h, pH 8.0, and the addition of 1% (w/v) maltose and 1% (w/v) beef extract to the culture medium. This enzyme was purified to homogeneity and had an apparent molecular mass of 41 kDa. The optimum pH range and temperature for the activity of phytase were found to be 7.0-8.0 and 60°C, respectively. This enzyme was strongly inhibited by 0.005 M of Mn(2+), Mg(2+), and Zn(2+). In vitro studies revealed that the phytase from Alcaligenes sp. released inorganic phosphate from plant phytates. Phytase released 1930 ± 28, 1740 ± 13, 1050 ± 31, 845 ± 7, 1935 ± 32, and 1655 ± 21 mg inorganic phosphate/kg plant phytates, namely, chick pea, corn, green pea, groundnut, pearl pea, and chick feed, respectively.
分离并鉴定了一株分泌植酸酶（EC 3.1.3.8）的细菌菌株，即产碱菌属（Alcaligenes sp.）。植酸酶生产的最佳条件包括发酵周期96小时、pH 8.0，以及在培养基中添加1%（w/v）麦芽糖和1%（w/v）牛肉浸膏。该酶被纯化至同质，表观分子量为41 kDa。发现植酸酶活性的最佳pH范围和温度分别为7.0 - 8.0和60°C。该酶受到0.005 M的Mn(2+)、Mg(2+)和Zn(2+)的强烈抑制。体外研究表明，产碱菌属的植酸酶能从植物肌醇六磷酸中释放无机磷酸盐。植酸酶分别从鹰嘴豆、玉米、青豆、花生、珍珠豆和鸡饲料等植物肌醇六磷酸中释放出1930±28、1740±13、1050±31、845±7、1935±32和1655±21毫克无机磷酸盐/千克植物肌醇六磷酸。
