Abiko Y, Hayakawa M, Aoki H, Saito S, Takiguchi H
Department of Biochemistry, Nihon University School of Dentistry, Chiba, Japan.
Arch Oral Biol. 1989;34(7):571-5. doi: 10.1016/0003-9969(89)90096-4.
A gene library of Strep. sobrinus B13N (serotype d) chromosomal DNA was constructed in Escherichia coli, with the bacteriophage vector lambda L47.1. A recombinant phage, lambda MDSM49, containing a 15.5 kb DNA insert, directed the expression of a 210 kDa antigenic protein. The recombinant 210 kDa protein was shown by Western blot analysis to be identical with cell-surface protein antigen A (spaA) from a serotype g strain. However, the restriction patterns of a subclone plasmid, pMD51, from lambda MDSM49 differed from those of serotype g strain. The cell-surface protein antigen I/II from serotype c Streptococcus mutans is a potential immunogen for vaccination against dental caries and corresponds to the spaA from serotype d and g strains. A recombinant clone, pDM51, will be a useful tool for serological and molecular biological studies. The recombinant spaA provides useful material for assessment of its diagnostic and immunogenic potential.
利用噬菌体载体λL47.1在大肠杆菌中构建了嗜酸性链球菌B13N（血清型d）染色体DNA基因文库。一个含有15.5 kb DNA插入片段的重组噬菌体λMDSM49指导表达一种210 kDa的抗原蛋白。通过蛋白质印迹分析表明，重组的210 kDa蛋白与血清型g菌株的细胞表面蛋白抗原A（spaA）相同。然而，来自λMDSM49的亚克隆质粒pMD51的限制性酶切图谱与血清型g菌株的不同。血清型c变形链球菌的细胞表面蛋白抗原I/II是预防龋齿疫苗的潜在免疫原，与血清型d和g菌株的spaA相对应。重组克隆pDM51将是血清学和分子生物学研究的有用工具。重组spaA为评估其诊断和免疫原性潜力提供了有用的材料。
