Takahashi I, Okahashi N, Sasakawa C, Yoshikawa M, Hamada S, Koga T
Department of Dental Research, National Institute of Health, Tokyo, Japan.
FEBS Lett. 1989 Jun 5;249(2):383-8. doi: 10.1016/0014-5793(89)80664-7.
The structural gene (pag gene) for a 210 kDa protein antigen of Streptococcus sobrinus serotype g was cloned and compared with that (pac gene) of a 190 kDa protein antigen of Streptococcus mutans serotype c. Immunodiffusion analysis revealed that the product of the pag gene immunologically cross-reacted with that of the pac gene. Southern blot and nucleotide sequence analyses revealed that a significant homology existed between the middle regions of the two structural genes.
克隆了远缘链球菌血清型g的210 kDa蛋白抗原的结构基因（pag基因），并将其与变形链球菌血清型c的190 kDa蛋白抗原的结构基因（pac基因）进行比较。免疫扩散分析表明，pag基因产物与pac基因产物发生免疫交叉反应。Southern印迹和核苷酸序列分析表明，两个结构基因的中间区域存在显著同源性。
