Chen Ruo-Wen, Liu Hui, An Jian-Xiong, Qian Xiao-Yan, Jiang Yi-De, Cope Doris K, Williams John P, Zhang Rui, Sun Li-Na
Department of Anesthesiology, Weifang Medical University, Weifang City, Shandong.
Department of Anesthesiology, Pain Medicine and Critical Care Medicine, Aviation General Hospital of China Medical University and Beijing Institute of Translational Medicine, Chinese Academy of Sciences, Beijing, China.
J Pain Res. 2017 Aug 8;10:1887-1897. doi: 10.2147/JPR.S140840. eCollection 2017.
The objective of this study was to investigate the effects of electro-acupuncture (EA) and pregabalin on cognition impairment induced by chronic trigeminal neuralgia (TN) in rats.
Controlled animal study.
Department of Anesthesiology, Pain Medicine and Critical Care Medicine, Aviation General Hospital of China Medical University.
Forty adult male Sprague Dawley rats.
Rats were randomly divided into four groups. The TN model was induced by administration of cobra venom to the left infraorbital nerve. On postoperative day 14, either EA or pregabalin was administered, free behavioral activities were observed. Spatial learning and memory abilities were determined in the Morris water maze. The ultrastructural alterations of the Gasserian ganglion, medulla oblongata and hippocampus were examined by electron microscopy. The changes on long-term potentiation were investigated.
After treatment, the exploratory behavior increased and the grooming behavior decreased (<0.05) for the EA group and pregabalin group compared with the cobra venom group; moreover, demyelination of neurons in Gasserian ganglion and medulla oblongata was reversed. The number of platform site crossings, the average percentages of time in the target quadrant and the field excitatory postsynaptic potential slopes increased (<0.05) in the EA group compared to the cobra venom group. However, the pregabalin group showed no differences compared to the cobra venom group (>0.05). Vacuolar degeneration in the hippocampal neurons was mild in the EA group, while it was severe in the pregabalin group.
EA and pregabalin could alleviate TN induced by cobra venom. EA could also inhibit the cognition deficit induced by TN, while pregabalin could not.
本研究旨在探讨电针（EA）和普瑞巴林对大鼠慢性三叉神经痛（TN）所致认知障碍的影响。
对照动物研究。
中国医科大学航空总医院麻醉科、疼痛医学与重症医学科。
40只成年雄性Sprague Dawley大鼠。
将大鼠随机分为四组。通过向左侧眶下神经注射眼镜蛇毒诱导TN模型。术后第14天，给予EA或普瑞巴林，观察自由行为活动。在Morris水迷宫中测定空间学习和记忆能力。通过电子显微镜检查三叉神经节、延髓和海马的超微结构改变。研究长时程增强的变化。
治疗后，与眼镜蛇毒组相比，EA组和普瑞巴林组的探究行为增加，梳理行为减少（P<0.05）；此外，三叉神经节和延髓神经元的脱髓鞘得到逆转。与眼镜蛇毒组相比，EA组的平台位点穿越次数、目标象限的平均时间百分比和场兴奋性突触后电位斜率增加（P<0.05）。然而，普瑞巴林组与眼镜蛇毒组相比无差异（P>0.05）。EA组海马神经元的空泡变性较轻，而普瑞巴林组则较重。
EA和普瑞巴林可减轻眼镜蛇毒诱导的TN。EA还可抑制TN诱导的认知缺陷，而普瑞巴林则不能。
