Hassfeld W, Steiner G, Hartmuth K, Kolarz G, Scherak O, Graninger W, Thumb N, Smolen J S
Second Department of Medicine, University of Vienna, Austria.
Arthritis Rheum. 1989 Dec;32(12):1515-20. doi: 10.1002/anr.1780321204.
Using immunoblot analysis with soluble nuclear extracts from HeLa cells, we identified autoantibodies to an antigen with a molecular weight of approximately 33,000 in 36% of 95 sera from rheumatoid arthritis patients, but in only 1 of 170 controls. The antigen, termed RA33, was resistant to DNase and RNase digestion but sensitive to proteinase K treatment. There was no discernible relation to other autoantibodies. Thus, this newly described autoantibody appears to be highly specific for rheumatoid arthritis.
通过对HeLa细胞核提取物进行免疫印迹分析，我们在95例类风湿性关节炎患者的血清中发现，36%的血清含有针对分子量约为33,000抗原的自身抗体，而在170例对照中只有1例含有该抗体。这种抗原被称为RA33，对DNA酶和RNA酶消化具有抗性，但对蛋白酶K处理敏感。它与其他自身抗体没有明显关系。因此，这种新描述的自身抗体似乎对类风湿性关节炎具有高度特异性。
