Ying Binbin, Maimaiti Abudu Kelimujiang, Song Donghui, Zhu Songsong
State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University Chengdu 610041, China ; Department of Stomatology, Ningbo First Hospital Ningbo 315000, China.
Department of Oral and Maxillofacial Surgery, First Teaching Hospital of Xinjiang Medical University Xinjiang 830000, China.
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1435-42. eCollection 2015.
The aim of this study is to evaluate the effects of myrtol standardized on cartilage lesions in osteoarthritis (OA) rats.
Fifty-six healthy Sprague-Dawley rats were randomly divided into sham group (13 rats) and OA model group (43 rats) with interior meniscus excision. Then serum estradiol (E2) and glycosaminoglycan (GAG) content in cartilage tissue were measured by radioimmunoassay and toluidine blue staining, respectively. After that, the model rats were randomly divided into low dose myrtol (LDM) group, middle dose myrtol (MDM) group and high dose myrtol (HDM) group (10 rats in each group) with treatment of 450 mg/kg, 300 mg/kg and 150 mg/kg myrtol, respectively. Then, Mankin scores were used to evaluate lesion extent of knee joint cartilage. Expression of tumor necrosis factor α (TNF-α), transforming growth factor β1 (TGF-β1), interleukin (IL)-6, Bax and Bcl-2 were investigated using PCR gel electrophoresis method.
Mankin cores were lower in sham group and myrtol group than in model group. There were statistically significant differences (P < 0.01) between sham group and model group in expression of TNF-α, TGF-β1, IL-6, Bax and Bcl-2 in the cartilage tissue. Myrtol significantly reduced the expression of TNF-α, IL-6 and Bax, and increased the expression of TGF-β1 and Bcl-2 in myrtol group, comparing with those in model group (P < 0.01).
Myrtol could down-regulate the expression of TNF-α, IL-6 and Bax, and up-regulate the expression of TGF-β1 and Bcl-2. Myrtol standardized is a promising drug to ameliorate knee cartilage lesions in the OA rat model.
本研究旨在评估标准桃金娘油对骨关节炎（OA）大鼠软骨损伤的影响。
将56只健康的Sprague-Dawley大鼠随机分为假手术组（13只大鼠）和内侧半月板切除术的OA模型组（43只大鼠）。然后分别通过放射免疫分析法和甲苯胺蓝染色法测定血清雌二醇（E2）和软骨组织中糖胺聚糖（GAG）的含量。之后，将模型大鼠随机分为低剂量桃金娘油（LDM）组、中剂量桃金娘油（MDM）组和高剂量桃金娘油（HDM）组（每组10只大鼠），分别给予450 mg/kg、300 mg/kg和150 mg/kg的桃金娘油进行治疗。然后，使用Mankin评分评估膝关节软骨的损伤程度。采用PCR凝胶电泳法研究肿瘤坏死因子α（TNF-α）、转化生长因子β1（TGF-β1）、白细胞介素（IL）-6、Bax和Bcl-2的表达。
假手术组和桃金娘油组的Mankin评分低于模型组。软骨组织中TNF-α、TGF-β1、IL-6、Bax和Bcl-2的表达在假手术组和模型组之间存在统计学显著差异（P < 0.01）。与模型组相比，桃金娘油组中桃金娘油显著降低了TNF-α、IL-6和Bax的表达，并增加了TGF-β1和Bcl-2的表达（P < 0.01）。
桃金娘油可下调TNF-α、IL-6和Bax的表达，并上调TGF-β1和Bcl-2的表达。标准桃金娘油是改善OA大鼠模型膝关节软骨损伤的一种有前景的药物。
