Geng Qian, Peng Haoyu, Chen Fengsheng, Luo Rongcheng, Li Rong
Cancer Center, Southern Medical University Guangzhou 510315, China ; Traditional Chinese Medicine-Integrated Hospital, Southern Medical University Guangzhou 510315, China.
Cancer Center, Southern Medical University Guangzhou 510315, China ; Department of Oncology, Nanfang Hospital, Southern Medical University Guangzhou 510515, China.
Int J Clin Exp Pathol. 2015 Feb 1;8(2):1938-45. eCollection 2015.
Sirt7, as one of the seven Sirtuin family members, which plays distinct roles in cancer progression, is bringing emerging attention due to its oncogenic characteristic. The expression of Sirt7 in breast cancer remained unclear, and the aim of this study was to elucidate its role in breast cancer.
A total of 188 cases included in this study were immunohistochemically evaluated for Sirt7, and western blot assay was used to assess its expression in breast cell lines as well as 36 breast cancer tissues and 36 paired non-cancerous tissues.
Upregulation of Sirt7 was found in breast cancer cell lines and breast cancer tissues (P < 0.001) by western blot analysis. Sirt7 was highly expressed in breast cancer tissue samples (67.8%) compared to adjacent normal breast tissues (31.8%) by immunohistochemical assay. It was also observed that the high expression level of Sirt7 was significantly correlated with high histological grade (P = 0.039) and negatively related to overall survival (P = 0.006). Sirt7 proved to be an independent prognostic factor (P = 0.007) in breast cancer.
Sirt7 expression was implicated with high histological grade and independently predicted poor clinical outcome in patients with breast cancer, suggesting that Sirt7 might play a role in the malignant progression of breast cancer.
Sirt7作为沉默调节蛋白家族的七个成员之一，在癌症进展中发挥着不同作用，因其致癌特性而受到越来越多的关注。Sirt7在乳腺癌中的表达尚不清楚，本研究旨在阐明其在乳腺癌中的作用。
本研究共纳入188例患者，采用免疫组织化学方法评估Sirt7的表达，并通过蛋白质印迹分析评估其在乳腺癌细胞系以及36例乳腺癌组织和36例配对的癌旁组织中的表达。
蛋白质印迹分析发现乳腺癌细胞系和乳腺癌组织中Sirt7表达上调（P < 0.001）。免疫组织化学分析显示，与邻近正常乳腺组织（31.8%）相比，Sirt7在乳腺癌组织样本中高表达（67.8%）。还观察到Sirt7的高表达水平与高组织学分级显著相关（P = 0.039），与总生存期呈负相关（P = 0.006）。Sirt7被证明是乳腺癌的独立预后因素（P = 0.007）。
Sirt7的表达与高组织学分级有关，并独立预测乳腺癌患者的不良临床结局，提示Sirt7可能在乳腺癌的恶性进展中起作用。
