Igci Mehri, Kalender Mehmet Emin, Borazan Ersin, Bozgeyik Ibrahim, Bayraktar Recep, Bozgeyik Esra, Camci Celaletdin, Arslan Ahmet
Gaziantep University, Faculty of Medicine, Department of Medical Biology, Gaziantep, Turkey.
Gaziantep University, Faculty of Medicine, Department of Oncology, Gaziantep, Turkey.
Gene. 2016 Jul 15;586(1):123-8. doi: 10.1016/j.gene.2016.04.023. Epub 2016 Apr 11.
Mammalian Sirtuins have been shown to perform distinct cellular functions and deregulated expression of these genes was reported to be involved in the development of various malignancies including breast cancer. An increasing number of evidence indicates that Sirtuins have both tumor promoter and tumor suppressor functions. However, the roles of Sirtuins have not been well-reported in breast cancer. In the present study, quantitative expression levels of Sirtuins (SIRT1-7) in breast cancer patients and breast cancer cell lines (MCF-7 and SKBR3) and control cell line (CRL-4010) were assessed by using a high-throughput real-time PCR method. As a result, Sirtuins were found to be differentially expressed in breast cancer tissues and cancer cell lines. Particularly, expressions of SIRT1 and SIRT4 were found to be significantly down-regulated in breast cancer tissues and SKBR3 breast cancer cells. In contrast, SIRT2, SIRT3, and SIRT5 genes were shown to be up-regulated in our study. Although SIRT6 and SIRT7 were also up-regulated in breast cancer tissues, these expression changes were statistically insignificant. Additionally, SIRT2, SIRT3, SIRT5, SIRT6 and SIRT7 were found to be differentially expressed in breast cancer cell lines. Yet, these changes were not well-correlated with tissue expression levels. In conclusion, Sirtuin family of genes shows differential expressions in breast cancer tissues and cells and SIRT1 and SIRT4 seem to play key tumor suppressor roles in breast cancer development. Herein, we report expression levels of Sirtuin family of genes in both breast cancer tissues and cancer cell lines simultaneously.
哺乳动物的沉默调节蛋白已被证明具有不同的细胞功能，据报道这些基因的表达失调与包括乳腺癌在内的各种恶性肿瘤的发生发展有关。越来越多的证据表明，沉默调节蛋白兼具肿瘤促进和肿瘤抑制功能。然而，沉默调节蛋白在乳腺癌中的作用尚未得到充分报道。在本研究中，我们采用高通量实时PCR方法评估乳腺癌患者、乳腺癌细胞系（MCF-7和SKBR3）以及对照细胞系（CRL-4010）中沉默调节蛋白（SIRT1-7）的定量表达水平。结果发现，沉默调节蛋白在乳腺癌组织和癌细胞系中存在差异表达。特别是，SIRT1和SIRT4的表达在乳腺癌组织和SKBR3乳腺癌细胞中显著下调。相比之下，在我们的研究中，SIRT2、SIRT3和SIRT5基因呈上调表达。虽然SIRT6和SIRT7在乳腺癌组织中也上调，但这些表达变化在统计学上无显著意义。此外，SIRT2、SIRT3、SIRT5、SIRT6和SIRT7在乳腺癌细胞系中存在差异表达。然而，这些变化与组织表达水平的相关性不佳。总之，沉默调节蛋白基因家族在乳腺癌组织和细胞中表现出差异表达，SIRT1和SIRT4似乎在乳腺癌发展中发挥关键的肿瘤抑制作用。在此，我们同时报道了沉默调节蛋白基因家族在乳腺癌组织和癌细胞系中的表达水平。
