Wu Gang, Guo Jing-Jing, Ma Zhen-Yu, Wang Jie, Zhou Zhong-Wen, Wang Yi
Depratment of General Surgery, Huashan Hospital, Fudan University Shanghai 200040, China.
Depratment of General Surgery, Huashan Hospital, Fudan University Shanghai 200040, China ; Depratment of General Surgery, Renhe Hospital Baoshan District, Shanghai 200431, China.
Int J Clin Exp Pathol. 2015 Feb 1;8(2):2010-7. eCollection 2015.
The correlation between calcification and papillary thyroid carcinoma has received increasing attention. We investigated the ability of bone sialoprotein (BSP) and osteopontin (OPN) protein levels to diagnose papillary thyroid carcinoma (PTC), and explored the correlation between BSP and OPN protein levels and calcification in PTC. Archival PTC specimens from patients with PTC with calcification and lateral cervical lymph node metastasis (LNM) were included in this retrospective immunohistochemical study. The protein levels of BSP and OPN were analysed immunohistochemically using routinely prepared tissue sections. PTC specimens from 66 patients with PTC were reviewed retrospectively (25 patients with histological calcification seen in paraffin sections, 41 patients without calcification; 35 patients with lateral cervical LNM, 31 patients without LNM). The percentage of samples that had cells that demonstrated positive protein staining differed significantly between PTC specimens, benign thyroid nodules, and adjacent normal follicular epithelium (BSP: 87.88%, 55.00%, and 42.50%, respectively; OPN: 83.33%, 70.00% and 50.00%, respectively). There was a significant difference in the immunohistochemical score (IHS) for BSP and OPN protein staining between PTC specimens with and without calcification (P < 0.05). The level of BSP protein staining was found to be significantly correlated with the level of OPN protein staining in PTC specimens. We conclude that the strong correlation between BSP and OPN and PTC suggests a role for BSP and OPN in calcification and tumor progression of PTC. BSP and OPN might be useful tumour markers for the diagnosis of PTC with limited value, because both of them had low specificity.
钙化与甲状腺乳头状癌之间的相关性已受到越来越多的关注。我们研究了骨唾液酸蛋白（BSP）和骨桥蛋白（OPN）蛋白水平诊断甲状腺乳头状癌（PTC）的能力，并探讨了PTC中BSP和OPN蛋白水平与钙化之间的相关性。本回顾性免疫组织化学研究纳入了伴有钙化和侧颈淋巴结转移（LNM）的PTC患者的存档PTC标本。使用常规制备的组织切片通过免疫组织化学分析BSP和OPN的蛋白水平。回顾性分析了66例PTC患者的PTC标本（25例石蜡切片中可见组织学钙化，41例无钙化；35例有侧颈LNM，31例无LNM）。PTC标本、良性甲状腺结节和相邻正常滤泡上皮中显示蛋白染色阳性的细胞样本百分比差异显著（BSP分别为87.88%、55.00%和42.50%；OPN分别为83.33%、70.00%和50.00%）。有钙化和无钙化的PTC标本之间BSP和OPN蛋白染色的免疫组织化学评分（IHS）存在显著差异（P<0.05）。发现PTC标本中BSP蛋白染色水平与OPN蛋白染色水平显著相关。我们得出结论，BSP和OPN与PTC之间的强相关性表明BSP和OPN在PTC的钙化和肿瘤进展中起作用。BSP和OPN可能是诊断PTC的有用肿瘤标志物，但价值有限，因为它们的特异性都较低。
