Guglielmotti María B, Domingo Mariela G, Steimetz Tammy, Ramos Emilio, Paparella María L, Olmedo Daniel G
Department of Oral Pathology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina.
National Research Council (CONICET), Buenos Aires, Argentina.
Eur J Oral Sci. 2015 Aug;123(4):242-8. doi: 10.1111/eos.12190. Epub 2015 May 14.
The aim of this experimental work was to evaluate deposition of titanium dioxide (TiO2 ) microparticles and nanoparticles, which could originate from titanium bioimplants, in the gingiva. Wistar rats were injected intraperitoneally (i.p.) with a suspension of TiO2 particles of different sizes (150, 10, or 5 nm). The rats were killed 12 months post-injection, and the buccal and lingual gingivae were resected and evaluated using light and scanning electron microscopy. Energy-dispersive X-ray spectroscopy (EDS) was used to confirm the presence of titanium in deposits of microparticles and nanoparticles, and the concentration of titanium in tissues was measured using inductively coupled plasma-mass spectrometry (ICP-MS). Histological examination showed that all experimental groups exhibited agglomerates, in the gingiva, of titanium particles of micrometer size range, with no associated inflammatory response. Higher concentrations of titanium traces were shown, by ICP-MS, in both buccal and lingual tissues of all experimental groups compared with their matched controls. Titanium concentrations were significantly higher in the buccal gingiva than in the lingual gingiva, and after injection with 5-nm particles than with 10-nm particles in both localizations. Titanium microparticles and nanoparticles deposit in the gingiva, and mostly on the buccal side. Gingival deposition of titanium could be considered a tissue indicator of tribocorrosion processes of titanium bioimplants.
这项实验工作的目的是评估可能源自钛生物植入物的二氧化钛（TiO₂）微粒和纳米颗粒在牙龈中的沉积情况。将不同尺寸（150、10或5纳米）的TiO₂颗粒悬浮液腹腔内（i.p.）注射到Wistar大鼠体内。在注射后12个月处死大鼠，切除颊侧和舌侧牙龈，并用光学显微镜和扫描电子显微镜进行评估。采用能量色散X射线光谱法（EDS）确认微粒和纳米颗粒沉积物中钛的存在，并使用电感耦合等离子体质谱法（ICP-MS）测量组织中钛的浓度。组织学检查显示，所有实验组的牙龈中均出现了微米尺寸范围的钛颗粒团聚物，且无相关炎症反应。ICP-MS结果显示，与匹配的对照组相比，所有实验组的颊侧和舌侧组织中钛痕量浓度均更高。颊侧牙龈中的钛浓度显著高于舌侧牙龈，且在两个部位，注射5纳米颗粒后的钛浓度均高于注射10纳米颗粒后的浓度。钛微粒和纳米颗粒沉积在牙龈中，且大多沉积在颊侧。钛在牙龈中的沉积可被视为钛生物植入物摩擦腐蚀过程的组织指标。
