Bruno Marcos E, Tasat Deborah R, Ramos Emilio, Paparella María L, Evelson Pablo, Rebagliati Raúl Jiménez, Cabrini Rómulo L, Guglielmotti María B, Olmedo Daniel G
Department of Oral Pathology, School of Dentistry, University of Buenos Aires, Buenos Aires, Argentina; School of Science and Technology, National University of San Martin, Buenos Aires, Argentina.
J Biomed Mater Res A. 2014 May;102(5):1439-48. doi: 10.1002/jbm.a.34822. Epub 2013 Jun 21.
Due to corrosion, a titanium implant surface can be a potential source for the release of micro (MPs) and nano-sized particles (NPs) into the biological environment. This work sought to evaluate the biokinetics of different sized titanium dioxide particles (TiO2 ) and their potential to cause cell damage. Wistar rats were intraperitoneally injected with 150 nm, 10 nm, or 5nm TiO2 particles. The presence of TiO2 particles was evaluated in histologic sections of the liver, lung, and kidney and in blood cells at 3 and 12 months. Ultrastructural analysis of liver and lung tissue was performed by TEM, deposit concentration in tissues was determined spectroscopically, and oxidative metabolism was assessed by determining oxidative membrane damage, generation of superoxide anion (O2(-)), and enzymatic and non-enzymatic antioxidants. TiO2 particles were observed inside mononuclear blood cells and in organ parenchyma at 3 and 12 months. TiO2 deposits were consistently larger in liver than in lung tissue. Alveolar macrophage O2(-) generation and average particle size correlated negatively (p < 0.05). NPs were more reactive and biopersistent in lung tissue than MPs. Antioxidant activity, particularly in the case of 5 nm particles, failed to compensate for membrane damage in liver cells; the damage was consistent with histological evidence of necrosis.
由于腐蚀，钛植入物表面可能会向生物环境中释放微米级（MPs）和纳米级颗粒（NPs）。这项工作旨在评估不同尺寸的二氧化钛颗粒（TiO₂）的生物动力学及其造成细胞损伤的可能性。将150纳米、10纳米或5纳米的TiO₂颗粒腹腔注射到Wistar大鼠体内。在3个月和12个月时，对肝脏、肺和肾脏的组织切片以及血细胞中的TiO₂颗粒进行评估。通过透射电子显微镜（TEM）对肝脏和肺组织进行超微结构分析，通过光谱法测定组织中的沉积物浓度，并通过测定氧化膜损伤、超氧阴离子（O₂⁻）的产生以及酶促和非酶促抗氧化剂来评估氧化代谢。在3个月和12个月时，在单核血细胞和器官实质中观察到了TiO₂颗粒。肝脏中的TiO₂沉积物始终比肺组织中的大。肺泡巨噬细胞O₂⁻的产生与平均颗粒大小呈负相关（p < 0.05）。纳米颗粒在肺组织中比微米颗粒更具反应性且生物持久性更强。抗氧化活性，尤其是5纳米颗粒的抗氧化活性，未能补偿肝细胞中的膜损伤；这种损伤与坏死的组织学证据一致。
