MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Biosens Bioelectron. 2015 Oct 15;72:114-20. doi: 10.1016/j.bios.2015.04.064. Epub 2015 Apr 21.
In this work, we report the first demonstration of chemiluminescence (CL) detection for microfluidic cloth-based analytical devices (μCADs). Wax screen-printing is used to make cloth channels or chambers, and enzyme-catalyzed CL reactions are imaged using an inexpensive charge coupled device (CCD). We first evaluate the relationship between the wicking rate and the length/width of cloth channel. For our device, the channel length and width between the loading and detection chambers are optimized to be 10mm and 3mm. Thus, the detection procedure can be accomplished in about 15s on a cloth-based device (15 × 30 mm(2)) by using 25-μL sample spotted on it. Next, several parameters affecting cloth-based CL intensity are studied, including exposure time, pH, and concentrations of luminol and enzyme. Under optimal conditions, a linear relationship is obtained between CL intensity and hydrogen peroxide (H2O2) concentrations in the range of 0.5-5mM with a detection limit of 0.46 mM. Finally, the utility of cloth-based CL is demonstrated for determination of H2O2 residues in meat samples. On our device, the chicken meat soaked for 6h with 3% H2O2 can be detected. Moreover, the supernatant of grinded meat sample can be directly applied, without need for other treatments. We believe that μCADs with CL detection could provide a new platform of rapid and low-cost assays for use in areas such as food detection and environmental monitoring.
在这项工作中，我们首次展示了基于微流控布基分析器件（μCAD）的化学发光（CL）检测。蜡质丝网印刷用于制作布质通道或腔室，并且使用廉价的电荷耦合器件（CCD）对酶促 CL 反应进行成像。我们首先评估了吸液速度与布质通道长度/宽度之间的关系。对于我们的设备，加载和检测腔室之间的通道长度和宽度优化为 10mm 和 3mm。因此，通过在布基器件（15×30mm²）上点样 25μL 的样品，大约 15s 就可以完成检测过程。接下来，研究了影响布基 CL 强度的几个参数，包括曝光时间、pH 值以及鲁米诺和酶的浓度。在最佳条件下，CL 强度与过氧化氢（H2O2）浓度在 0.5-5mM 范围内呈线性关系，检测限为 0.46mM。最后，展示了基于布基 CL 的用于测定肉样中 H2O2 残留的应用。在我们的设备上，可以检测到用 3%H2O2 浸泡 6h 的鸡肉。此外，无需其他处理，可直接应用研磨肉样的上清液。我们相信，具有 CL 检测功能的 μCAD 可为食品检测和环境监测等领域提供快速、低成本分析的新平台。
