Department of Chemistry, Kyungpook National University, Daegu 702-701, South Korea.
Anal Bioanal Chem. 2012 Dec;404(10):3165-73. doi: 10.1007/s00216-012-6429-1. Epub 2012 Oct 4.
Chemiluminescence (CL) emission from luminol-tetrachloroaurate (AuCl(4)) system studied in presence of monosaccharide sugars such as glucose and fructose was investigated on a microfluidic chip fabricated by the soft lithography technique. CL emission from the luminol-AuCl(4) system at 430 nm was intensified remarkably by the catalytic activity of glucose and fructose at room temperature. Under optimized conditions, the CL emission intensity of the system was found to be linearly related to the concentration of the sugars. Based on this observation, nonenzymatic determination of total sugar (glucose, fructose, or hydrolyzable sucrose) was performed in a rapid and sensitive analytical method. The results revealed that the linearity ranged from 9 to 1,750 μM for glucose and 80 to 1,750 μM for fructose, with a limit of detection of 0.65 and 0.69 μM, respectively. The relative standard deviations determined at 250 μM based on six repetitive injections were 1.13 and 1.15% for glucose and fructose, respectively. The developed method was successfully applied for determination of the total sugar concentration in food and beverages.
在微流控芯片上通过软光刻技术制备后，研究了在单糖（如葡萄糖和果糖）存在下，鲁米诺-四氯金酸（AuCl(4))体系的化学发光（CL）发射。在室温下，葡萄糖和果糖的催化活性显著增强了鲁米诺-AuCl(4)体系在 430nm 处的 CL 发射。在优化条件下，发现该体系的 CL 发射强度与糖的浓度呈线性关系。基于这一观察，采用快速灵敏的分析方法，对总糖（葡萄糖、果糖或可水解蔗糖）进行了非酶法测定。结果表明，葡萄糖的线性范围为 9 至 1750μM，果糖的线性范围为 80 至 1750μM，检测限分别为 0.65 和 0.69μM。基于 6 次重复进样，在 250μM 时葡萄糖和果糖的相对标准偏差分别为 1.13%和 1.15%。该方法成功应用于食品和饮料中总糖浓度的测定。
