Liu Wei, Guo Yumei, Li Huifang, Zhao Mei, Lai Zesheng, Li Baoxin
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 25;137:1298-303. doi: 10.1016/j.saa.2014.09.059. Epub 2014 Sep 28.
Paper-based devices are biodegradable and have been used in diagnosis and environmental analysis field. In this work, a wax-printed paper-based analytical device combined with silver nanoparticles (AgNPs) catalyzed luminol chemiluminescence (CL) system for the determination of ofloxacin (OFLX) was presented. It was based on the enhancement of CL intensity of luminol-H2O2-OFLX system by AgNPs. Wax-printing fabrication technique was used to make the simple circle shaped paper device and large scale chips can be fabricated at the same time. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the concentration of OFLX in the range from 1.0×10(-9) g/mL to 1.0×10(-6) g/mL with a detection limit of 3.0×10(-10) g/mL. This method has been successfully applied to the determination of OFLX in eyedrop samples.
纸质设备具有生物可降解性，已被应用于诊断和环境分析领域。在本工作中，提出了一种结合银纳米颗粒（AgNPs）催化鲁米诺化学发光（CL）体系的蜡印纸质分析装置，用于测定氧氟沙星（OFLX）。它基于AgNPs对鲁米诺-H2O2-OFLX体系CL强度的增强作用。采用蜡印制造技术制作简单的圆形纸质装置，并且可以同时制造大规模芯片。在选定的实验条件下，CL强度与OFLX浓度在1.0×10(-9) g/mL至1.0×10(-6) g/mL范围内呈线性关系，检测限为3.0×10(-10) g/mL。该方法已成功应用于滴眼液样品中OFLX的测定。
