Marozsán-Tóth Zsuzsa, Vashegyi Ildikó, Galiba Gábor, Tóth Balázs
Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Department of Plant Molecular Biology, H-2462 Martonvásár, Brunszvik u. 2, Hungary.
J Plant Physiol. 2015 Jun 1;181:42-9. doi: 10.1016/j.jplph.2015.04.002. Epub 2015 Apr 17.
Cold acclimation ability is crucial in the winter survival of cereals. In this process CBF transcription factors play key role, therefore understanding the regulation of these genes might provide useful knowledge for molecular breeding. In the present study the signal transduction pathways leading to the cold induction of different CBF genes were investigated in barley cv. Nure using pharmacological approach. Our results showed that the cold induced expression of CBF9 and CBF14 transcription factors is regulated by phospholipase C, phospholipase D pathways and calcium. On the contrary, these pathways have negative effect on the cold induction of CBF12 that is regulated by a different, as yet unidentified pathway. The diversity in the regulation of these transcription factors corresponds to their sequence based phylogenetic relationships suggesting that their evolutionary separation happened on structural, functional and regulational levels as well. On the CBF effector gene level, the signaling regulation is more complex, resultant effect of multiple pathways.
冷驯化能力对于谷物在冬季的存活至关重要。在此过程中，CBF转录因子发挥着关键作用，因此了解这些基因的调控机制可能为分子育种提供有用的知识。在本研究中，采用药理学方法在大麦品种Nure中研究了导致不同CBF基因冷诱导的信号转导途径。我们的结果表明，CBF9和CBF14转录因子的冷诱导表达受磷脂酶C、磷脂酶D途径和钙的调控。相反，这些途径对由不同的、尚未确定的途径调控的CBF12的冷诱导具有负面影响。这些转录因子调控的多样性与其基于序列的系统发育关系相对应，表明它们在结构、功能和调控水平上也发生了进化分离。在CBF效应基因水平上，信号调控更为复杂，是多种途径的综合结果。
