Kitabatake Takae Tamy, Marini Luana de Castro, Gonçalves Renata Batagini, Bertolino Guilherme, de Souza Hugo Celso Dutra, de Araujo João Eduardo
Laboratory of Neuropsychobiology and Motor Behavior, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School of the University of São Paulo (USP), AV. dos Bandeirantes, 3900, Ribeirão Preto (SP), 14049-900, Brazil.
Laboratory of Neuropsychobiology and Motor Behavior, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School of the University of São Paulo (USP), AV. dos Bandeirantes, 3900, Ribeirão Preto (SP), 14049-900, Brazil.
Behav Brain Res. 2015 Sep 15;291:20-25. doi: 10.1016/j.bbr.2015.04.057. Epub 2015 May 11.
This study aims to investigate the effects of treadmill training and the possible influences of the start, regularity, and duration after experimental ischemic stroke. We performed bilateral occlusion of both the carotid arteries for 5 min in Mongolian gerbils. The training groups were: continuous training for 5 consecutive days or not continuous training for 3 non-consecutive days. The groups remained in the treadmill for 15 min, and the training started 12 or 24h after the stroke. In the activity cage (AC), behaviors of crossing and distance traveled were registered. The forced motor behavior was analyzed by a Rota Rod test. After the experiments, the brains were perfused, and coronal sections of the striatum, motor cortex M1, and CA1 area of the hippocampus were cut and stained with hematoxylin and eosin. Behavioral and morphological data analysis was performed by an ANOVA and MANCOVA. Results showed an increased density of neurons in the CA1 area of the hippocampus, motor cortex M1, and the striatum in ischemic training groups compared to the not training ischemic groups. Animals subjected to continuous training that started 24h after ischemia showed a satisfactory behavior in the AC and a higher number of striatal neurons when compared to ischemic animals. The animals in the not continuous training started 12h after the stroke showed an unsatisfactory performance in the AC and Rota Rod. These findings suggest that early training and not continuous training are inadequate for motor behavior improvement after stroke.
本研究旨在探讨跑步机训练的效果以及实验性缺血性中风后开始训练的时间、训练的规律性和持续时间可能产生的影响。我们对蒙古沙鼠的双侧颈动脉进行了5分钟的闭塞。训练组分为：连续5天进行持续训练或3天非连续进行非持续训练。各组在跑步机上停留15分钟，训练在中风后12或24小时开始。在活动笼（AC）中，记录穿越行为和行进距离。通过转棒试验分析强迫运动行为。实验结束后，对大脑进行灌注，切取纹状体、运动皮层M1和海马CA1区的冠状切片，并用苏木精和伊红染色。通过方差分析和多变量协方差分析进行行为和形态学数据分析。结果显示，与未训练的缺血组相比，缺血训练组海马CA1区、运动皮层M1和纹状体中的神经元密度增加。与缺血动物相比，缺血后24小时开始连续训练的动物在活动笼中表现出令人满意的行为，纹状体神经元数量更多。中风后12小时开始非连续训练的动物在活动笼和转棒试验中表现不佳。这些发现表明，早期训练和非连续训练对中风后运动行为的改善是不够的。
