Laboratory of Neuropsychobiology and Motor Behavior, Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, Ribeirão Preto Medical School of the University of São Paulo (USP), São Paulo, Brazil.
Int J Radiat Biol. 2013 Aug;89(8):595-601. doi: 10.3109/09553002.2013.784422. Epub 2013 Apr 16.
To evaluate the effects of continuous magnetic field stimulation after four days of global encephalic ischemia on motor behavior and brain morphology in gerbils.
Sixty Mongolian gerbils were divided into five groups: Control (CG), sham (SG), ischemia (IG), ischemia north (INPG) and south (ISPG) pole. Experimental animals underwent bilateral occlusion of the carotid artery. In groups with magnetic stimulation, a 3200 gauss magnet was fixed to the skull. After brain perfusion, coronal sections of the striatum, motor cortex (M1) and CA1 area of the hippocampus (CA1) were cut and stained with hematoxylin and eosin.
In the open field test (OF) we observed an increase in crossing in the IG compared to the CG, INPG and ISPG (F = 20.19), and a reduction in the time spent on the Rota-rod test (RR) with the IG compared to CG, IPNG and ISPG (F = 17.59). Morphometric analysis revealed a reduction in the density of the neurons in the CA1, in the number of M1 and striatal neurons in the IG compared to the CG, INPG and ISPG.
Our results demonstrate a potential therapeutic application of static magnetic fields for the preservation of motor behavior and neurons in regions analyzed after global cerebral ischemia.
评估在全脑缺血 4 天后对连续磁场刺激对沙土鼠运动行为和脑形态的影响。
将 60 只蒙古沙土鼠分为 5 组：对照组（CG）、假手术组（SG）、缺血组（IG）、北磁极刺激组（INPG）和南磁极刺激组（ISPG）。实验动物行双侧颈总动脉结扎。在磁场刺激组，用 3200 高斯的磁铁固定在颅骨上。脑灌注后，取纹状体、运动皮质（M1）和海马 CA1 区冠状切片，用苏木精和伊红染色。
在旷场试验（OF）中，我们观察到与 CG、INPG 和 ISPG 相比，IG 组的穿越次数增加（F = 20.19），与 CG、IPNG 和 ISPG 相比，IG 组在转棒试验（RR）中的时间减少（F = 17.59）。形态计量学分析显示，与 CG、INPG 和 ISPG 相比，IG 组的 CA1 神经元密度、M1 和纹状体神经元数量减少。
我们的结果表明，静磁场对全脑缺血后分析的运动行为和神经元具有潜在的治疗应用。
