Zhang Lurong, Jiang Guorong, Yao Fei, Liang Guoqiang, Wang Fei, Xu Heng, Wu Yan, Yu Xiao, Liu Haiyan
Laboratory of Cellular and Molecular Tumor Immunology, Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University , Suzhou , P.R. China .
Immunopharmacol Immunotoxicol. 2015 Jun;37(3):301-7. doi: 10.3109/08923973.2015.1035391. Epub 2015 May 15.
Osthole, a natural coumarin derivative, has been shown to have anti-tumor and anti-inflammatory activity. However, the effect of osthole on anti-tumor immune responses in tumor-bearing mice has not yet been reported. In the present study, osthole treatment did not affect the weight and the coefficient of thymus and spleen in tumor-bearing mice with hepatocellular carcinoma (HCC). However, osthole administration significantly elevated the proportion and number of the splenic CD8(+) T cells, the proportion of CD4(+) T and CD8(+) T cells in tumor tissues, and the levels of IL-2 and TNF-α in the serum of HCC tumor-bearing mice. Our results suggested that osthole could promote the activation of the tumor-infiltrating CD4(+) T and CD8(+) T cells, and elevate the proportion of CD4(+) and CD8(+) effector T cells. Osthole treatment also significantly decreased the proportion of CD4(+)CD25(+)Foxp3(+) regulatory T cells in the spleen. Taken together, osthole could enhance the T cell mediated anti-tumor immune responses in the tumor-bearing mice with HCC.
蛇床子素是一种天然香豆素衍生物，已被证明具有抗肿瘤和抗炎活性。然而，蛇床子素对荷瘤小鼠抗肿瘤免疫反应的影响尚未见报道。在本研究中，蛇床子素处理对荷肝癌（HCC）小鼠的体重以及胸腺和脾脏系数没有影响。然而，给予蛇床子素显著提高了荷HCC肿瘤小鼠脾脏中CD8(+) T细胞的比例和数量、肿瘤组织中CD4(+) T细胞和CD8(+) T细胞的比例以及血清中IL-2和TNF-α的水平。我们的结果表明，蛇床子素可以促进肿瘤浸润性CD4(+) T细胞和CD8(+) T细胞的活化，并提高CD4(+)和CD8(+)效应T细胞的比例。蛇床子素处理还显著降低了脾脏中CD4(+)CD25(+)Foxp3(+)调节性T细胞的比例。综上所述，蛇床子素可以增强荷HCC肿瘤小鼠中T细胞介导的抗肿瘤免疫反应。
