Unciti-Broceta Juan D, Cano-Cortés Victoria, Altea-Manzano Patricia, Pernagallo Salvatore, Díaz-Mochón Juan J, Sánchez-Martín Rosario M
1] Pfizer - Universidad de Granada - Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, 18016 Granada, Spain [2] NanoGetic S. L. Parque Tecnológico Ciencias de la Salud (PTS), Avenida de la Innovación 1, Edificio BIC, 18016 Armilla - Granada (Spain).
Pfizer - Universidad de Granada - Junta de Andalucía Centre for Genomics and Oncological Research (GENYO), Parque Tecnológico de Ciencias de la Salud (PTS), Avenida de la Ilustración 114, 18016 Granada, Spain.
Sci Rep. 2015 May 15;5:10091. doi: 10.1038/srep10091.
Engineered nanoparticles (eNPs) for biological and biomedical applications are produced from functionalised nanoparticles (NPs) after undergoing multiple handling steps, giving rise to an inevitable loss of NPs. Herein we present a practical method to quantify nanoparticles (NPs) number per volume in an aqueous suspension using standard spectrophotometers and minute amounts of the suspensions (up to 1 μL). This method allows, for the first time, to analyse cellular uptake by reporting NPs number added per cell, as opposed to current methods which are related to solid content (w/V) of NPs. In analogy to the parameter used in viral infective assays (multiplicity of infection), we propose to name this novel parameter as multiplicity of nanofection.
用于生物和生物医学应用的工程纳米颗粒（eNPs）是由功能化纳米颗粒（NPs）经过多个处理步骤后产生的，这不可避免地导致了纳米颗粒的损失。在此，我们提出了一种实用方法，可使用标准分光光度计和微量悬浮液（最多1 μL）来量化水悬浮液中每体积的纳米颗粒（NPs）数量。该方法首次能够通过报告每个细胞添加的纳米颗粒数量来分析细胞摄取情况，这与当前与纳米颗粒的固体含量（w/V）相关的方法不同。类似于病毒感染测定中使用的参数（感染复数），我们建议将这个新参数命名为纳米转染复数。
