Ons Sheila, Lavore Andrés, Sterkel Marcos, Wulff Juan Pedro, Sierra Ivana, Martínez-Barnetche Jesús, Rodriguez Mario Henry, Rivera-Pomar Rolando
Laboratorio de Genética y Genómica Funcional, Centro Regional de Estudios Genómicos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Bvd 120 y 62, La Plata, Buenos Aires, Argentina.
Departamento de Ciencias Básicas y Experimentales, Universidad Nacional del Noroeste de la Provincia de Buenos Aires, Monteagudo 2772, 2700 Pergamino, Buenos Aires, Argentina.
Insect Biochem Mol Biol. 2016 Feb;69:34-50. doi: 10.1016/j.ibmb.2015.05.003. Epub 2015 May 12.
The importance of Chagas disease motivated the scientific effort to obtain the complete genomic sequence of the vector species Rhodnius prolixus, this information is also relevant to the understanding of triatomine biology in general. The central nervous system is the key regulator of insect physiology and behavior. Neurohormones (neuropeptides and biogenic amines) are the chemical messengers involved in the regulation and integration of neuroendocrine signals. In insects, this signaling is mainly mediated by the interaction of neurohormone ligands with G protein coupled receptors (GPCRs). The recently sequenced R. prolixus genome provides us with the opportunity to analyze this important family of genes in triatomines, supplying relevant information for further functional studies. Next-generation sequencing methods offer an excellent opportunity for transcriptomic exploration in key organs and tissues in the presence of a reference genome as well as when a reference genome is not available. We undertook a genomic analysis to obtain a genome-wide inventory of opsines and the GPCRs for neurohormones in R. prolixus. Furthermore, we performed a transcriptomic analysis of R. prolixus central nervous system, focusing on neuropeptide precursor genes and neurohormone and opsines GPCRs. In addition, we mined the whole transcriptomes of Triatoma dimidiata, Triatoma infestans and Triatoma pallidipennis - three sanitary relevant triatomine species - to identify neuropeptide precursors and GPCRs genes. Our study reveals a high degree of sequence conservation in the molecular components of the neuroendocrine system of triatomines.
恰加斯病的重要性促使科学界努力获取病媒物种长红猎蝽的完整基因组序列，这些信息对于全面了解锥蝽生物学也具有重要意义。中枢神经系统是昆虫生理和行为的关键调节者。神经激素（神经肽和生物胺）是参与神经内分泌信号调节和整合的化学信使。在昆虫中，这种信号传导主要由神经激素配体与G蛋白偶联受体（GPCRs）的相互作用介导。最近测序的长红猎蝽基因组为我们提供了分析锥蝽中这个重要基因家族的机会，为进一步的功能研究提供了相关信息。下一代测序方法为在有参考基因组以及没有参考基因组的情况下对关键器官和组织进行转录组学探索提供了绝佳机会。我们进行了一项基因组分析，以获得长红猎蝽中视蛋白和神经激素GPCRs的全基因组清单。此外，我们对长红猎蝽的中枢神经系统进行了转录组分析，重点关注神经肽前体基因以及神经激素和视蛋白GPCRs。另外，我们挖掘了二点锥蝽、侵扰锥蝽和苍白锥蝽这三种与卫生相关的锥蝽物种的全转录组，以鉴定神经肽前体和GPCRs基因。我们的研究揭示了锥蝽神经内分泌系统分子成分中的高度序列保守性。
