Takahashi Yoshitaka, Otsuki Akemi, Mori Yoshiko, Kawakami Yuki, Ito Hideyuki
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan.
Food Chem. 2015 Nov 1;186:2-5. doi: 10.1016/j.foodchem.2015.03.105. Epub 2015 Mar 30.
Oxidation of low-density lipoprotein (LDL) is one of the crucial steps for atherosclerosis development, and an essential role of leukocyte-type 12-lipoxygenase expressed in macrophages in this process has been demonstrated. The biochemical mechanism of the oxidation of circulating LDL by leukocyte-type 12-lipoxygenase in macrophages has been proposed. The major ingredients in guava tea leaves which inhibited the catalytic activity of leukocyte-type 12-lipoxygenase were quercetin and ethyl gallate. Administration of extracts from guava tea leaves to apoE-deficient mice significantly attenuated atherogenic lesions in the aorta and aortic sinus. We recently showed that Qing Shan Lu Shui inhibited the catalytic activity of leukocyte-type 12-lipoxygenase. The major components inhibiting the enzyme contained in Qing Shan Lu Shui were identified to be novel monoterpene glycosides. The anti-atherogenic effect of the tea leaves might be attributed to the inhibition of leukocyte-type 12-lipoxygenase by these components.
低密度脂蛋白（LDL）的氧化是动脉粥样硬化发展的关键步骤之一，并且已经证明巨噬细胞中表达的白细胞型12-脂氧合酶在此过程中起着重要作用。已经提出了巨噬细胞中白细胞型12-脂氧合酶氧化循环LDL的生化机制。番石榴茶叶中抑制白细胞型12-脂氧合酶催化活性的主要成分是槲皮素和没食子酸乙酯。给载脂蛋白E缺陷小鼠施用番石榴茶叶提取物可显著减轻主动脉和主动脉窦中的动脉粥样硬化病变。我们最近发现青山绿水抑制白细胞型12-脂氧合酶的催化活性。经鉴定，青山绿水中抑制该酶的主要成分是新型单萜糖苷。茶叶的抗动脉粥样硬化作用可能归因于这些成分对白细胞型12-脂氧合酶的抑制作用。
