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睡莲花朵茶汤中黄酮类化合物和挥发性化合物的评估及其抗氧化活性

Assessment of flavonoids and volatile compounds in tea infusions of water lily flowers and their antioxidant activities.

作者信息

Yin Dan-Dan, Yuan Ru-Yu, Wu Qian, Li Shan-Shan, Shao Shuai, Xu Yan-Jun, Hao Xiang-Hong, Wang Liang-Sheng

机构信息

Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; College of Horticulture, Nanjing Agriculture University, Nanjing 210095, China.

出版信息

Food Chem. 2015 Nov 15;187:20-8. doi: 10.1016/j.foodchem.2015.04.032. Epub 2015 Apr 18.

Abstract

Water lily, a member of the Nymphaeaceae family, can be made into tea on the basis of outstanding fragrance characteristics and health care functions. In this study, 16 flavonoids were identified and quantified in tea infusions prepared from the petals of 33 water lily cultivars using HPLC-DAD and HPLC-ESI-MS/MS. The infusions were analyzed with HS-SPME coupled with GC-MS; 29 volatile compounds were detected, of which nine were found to be scent components. The cultivars were clustered into three clusters characterized according to scent components. The 'Conqueror' and 'Virginia' cultivars had the highest antioxidant activities. The concentrations of polyphenols and flavonoids showed significant positive correlations with antioxidant activity as measured by DPPH, ABTS(+), and FRAP assays. This study is valuable for a fuller understanding of this important tea and can also be used for the development of water lily.

摘要

睡莲是睡莲科的一员,基于其出色的香气特性和保健功能可制成茶。在本研究中,使用HPLC-DAD和HPLC-ESI-MS/MS对33个睡莲品种花瓣制成的茶汤中的16种黄酮类化合物进行了鉴定和定量。采用HS-SPME与GC-MS联用对茶汤进行分析;检测到29种挥发性化合物,其中9种为香气成分。根据香气成分,这些品种被聚类为三个簇。“征服者”和“弗吉尼亚”品种具有最高的抗氧化活性。通过DPPH、ABTS(+)和FRAP测定法测得,多酚和黄酮类化合物的浓度与抗氧化活性呈显著正相关。本研究对于更全面地了解这种重要的茶具有重要价值,也可用于睡莲的开发。

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