Stonans I Ia, Gorlina N K, Cheredeev A N, Bogdanov M E, Khoperskaia O A, Kushnir N V, Kozlov I G
Biull Eksp Biol Med. 1989 Oct;108(10):473-5.
Normalizing influence of different lymphocyte populations and their soluble factors on L-929 transformed malignant fibroblasts (L cells) has been examined. It was demonstrated that splenic T-lymphocytes caused stable (heritable) normalization of receptor apparatus, biophysical and proliferative characteristics of L cells. Lymphokine with the activity of normalization factor (NF) was purified from 24-hour immunocyte conditioned medium. Stability of the normalization phenomenon was caused by NF induced synthesis of functionally analogous factor in L cells. The results obtained indicate the existence of non-cytotoxic mechanisms of tumour growth immunological control. The isolated lymphokine possessed also the activity of early embryonal cell differentiation factor. It is suggested that lymphokines with the activity of NF are physiological regulators of nonlymphoid cell differentiation.
研究了不同淋巴细胞群体及其可溶性因子对L-929转化恶性成纤维细胞（L细胞）的正常化影响。结果表明，脾T淋巴细胞可使L细胞的受体装置、生物物理和增殖特性实现稳定（可遗传）的正常化。从24小时免疫细胞条件培养基中纯化出具有正常化因子（NF）活性的淋巴因子。正常化现象的稳定性是由NF诱导L细胞合成功能类似因子所致。所得结果表明存在肿瘤生长免疫控制的非细胞毒性机制。分离出的淋巴因子还具有早期胚胎细胞分化因子的活性。提示具有NF活性的淋巴因子是非淋巴细胞分化的生理调节因子。
