Wu Xiaoling, Ge Jun, Zhu Jingying, Zhang Yifei, Yong You, Liu Zheng
Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Chem Commun (Camb). 2015 Jun 14;51(47):9674-7. doi: 10.1039/c5cc01776a.
Using aldehyde-functionalized Pluronic as the reactive surfactant, enzyme-Pluronic conjugates with sizes ranging from nanometers to micrometers were synthesized in reverse emulsions. Compared with the direct conjugation in aqueous solution, this method gave an increased conjugation efficiency and well-controlled size of the conjugates. The versatility of this method was validated using horseradish peroxidase (HRP), Candida rugosa lipase (CRL) and Candida antarctica lipase B (CALB). The resulting enzyme-Pluronic conjugates showed greatly enhanced apparent activity compared to free enzymes in organic media.
以醛基功能化的普朗尼克作为反应性表面活性剂，在反相乳液中合成了尺寸范围从纳米到微米的酶 - 普朗尼克缀合物。与在水溶液中的直接缀合相比，该方法提高了缀合效率并能很好地控制缀合物的尺寸。使用辣根过氧化物酶（HRP）、皱落假丝酵母脂肪酶（CRL）和南极假丝酵母脂肪酶B（CALB）验证了该方法的通用性。与有机介质中的游离酶相比，所得的酶 - 普朗尼克缀合物表现出明显增强的表观活性。
