†Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), Corner College and Cooper Roads (Bldg 75), and ‡School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane QLD 4072, Australia.
ACS Nano. 2015 Jun 23;9(6):6354-62. doi: 10.1021/acsnano.5b01929. Epub 2015 May 22.
A rapid and simple approach is presented to address two critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore-integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD)-induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay-based devices. These nanoscaled physical forces acting within nanometer distances from the electrode surface enabled rapid (40 min), sensitive (10 fg/mL), and highly specific detection of human epidermal growth factor receptor 2 in breast cancer patient samples. We believe this approach presents potential for the development of rapid and sensitive SERS immunoassays for routine clinical diagnosis.
提出了一种快速而简单的方法来解决基于表面增强拉曼散射（SERS）的免疫分析中的两个关键问题，如去除/避免非特异性吸附和缩短分析时间。所展示的方法涉及合理设计的荧光染料集成金/银纳米壳作为 SERS 纳米标签，并利用交流电流电动流体动力学（ac-EHD）诱导的纳米级表面剪切力来增强捕获动力学。通过与流体动力学流动和传统基于免疫测定的设备进行比较来验证该测定的性能。这些纳米级物理力在离电极表面纳米距离内作用，能够快速（40 分钟）、灵敏（10 fg/mL）和高度特异性地检测乳腺癌患者样本中的人表皮生长因子受体 2。我们相信这种方法为快速灵敏的 SERS 免疫分析的常规临床诊断提供了潜力。
