中国河南麋鹿（Elaphurus davidianus）中的人兽共患微小隐孢子虫基因型
Zoonotic Enterocytozoon bieneusi genotypes in Pere David's deer (Elaphurus davidianus) in Henan, China.
作者信息
Zhang Zhenjie, Huang Jianying, Karim Md Robiul, Zhao Jinfeng, Dong Haiju, Ai Weichang, Li Fuhuang, Zhang Longxian, Wang Rongjun
机构信息
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
The Wild Animals Rescue Center of Henan Province, Zhengzhou 450044, China.
出版信息
Exp Parasitol. 2015 Aug;155:46-8. doi: 10.1016/j.exppara.2015.05.008. Epub 2015 May 14.
Enterocytozoon bieneusi is a zoonotic pathogen of the phylum Microspora that infects humans as well as a variety of animal species worldwide. While molecular epidemiologic studies have characterized this parasite in various hosts, isolates from many susceptible hosts have not yet been examined. In this study, E. bieneusi was isolated from 47 Pere David's deer (Elaphurus davidianus) in Henan, China and characterized via PCR analysis of the internal transcribed spacer (ITS) gene. E. bieneusi was detected in 16 out of 47 (34.0%) fecal specimens examined. Sequence analysis of the ITS revealed six known genotypes: type IV (4), EbpC (4), EbpA (4), BEB6 (2), COS-I (1), and COS-II (1). Of these, type IV, EbpC, and EbpA are known to cause human microsporidiosis worldwide, whereas the remaining genotypes are generally specific to ruminants. The present study indicated that Pere David's deer are naturally infected with E. bieneusi, predominantly with zoonotic genotypes, and may pose a risk for human transmission.
微小隐孢子虫是微孢子虫门的一种人畜共患病原体，可感染全球范围内的人类以及多种动物物种。虽然分子流行病学研究已对该寄生虫在各种宿主中的情况进行了表征，但来自许多易感宿主的分离株尚未得到检测。在本研究中，从中国河南的47只麋鹿（Elaphurus davidianus）中分离出微小隐孢子虫，并通过对内部转录间隔区（ITS）基因进行PCR分析对其进行表征。在所检测的47份粪便样本中，有16份（34.0%）检测到微小隐孢子虫。ITS序列分析显示有六种已知基因型：IV型（4份）、EbpC（4份）、EbpA（4份）、BEB6（2份）、COS-I（1份）和COS-II（1份）。其中，IV型、EbpC和EbpA在全球范围内已知可导致人类微孢子虫病，而其余基因型通常对反刍动物具有特异性。本研究表明，麋鹿自然感染了微小隐孢子虫，主要是感染了人畜共患基因型，可能对人类传播构成风险。
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