基于蛋白质稳定的金纳米簇用于牛奶中氯霉素检测的荧光猝灭法。
Fluorescence quenching for chloramphenicol detection in milk based on protein-stabilized Au nanoclusters.
作者信息
Tan Zhijing, Xu Hua, Li Gu, Yang Xiupei, Choi Martin M F
机构信息
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, PR China.
College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong 637000, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2015;149:615-20. doi: 10.1016/j.saa.2015.04.109. Epub 2015 May 8.
In the present study, we report a simple and rapid method for sensitive and selective determination of chloramphenicol (CAP) based on fluorescence of bovine serum albumin-stabilized Au nanoclusters (BSA-AuNCs). The BSA-AuNCs exhibit strong red emission. Upon addition of CAP to BSA-AuNCs, the fluorescence intensity of AuNCs shows a dramatic decrease attributing to the photo-induced electron transfer process from the electrostatically attached CAP to the BSA-AuNCs. The effects of pH, amount of BSA-AuNCs, temperature and reaction time on the detection of chloramphenicol were investigated. Under the optimal conditions, trace amounts of CAP could be detected. The linear working range is 0.10-70.00 μM with a detection limit 33 nM (S/N=3). In addition, the proposed method has been successfully applied to the detection of CAP in milk samples and largely improves the application of spectral method for quantitative analysis of CAP.
在本研究中，我们报道了一种基于牛血清白蛋白稳定的金纳米簇（BSA-AuNCs）荧光的灵敏且选择性测定氯霉素（CAP）的简单快速方法。BSA-AuNCs呈现出强烈的红色发射。向BSA-AuNCs中加入CAP后，金纳米簇的荧光强度显著降低，这归因于通过静电作用附着的CAP向BSA-AuNCs的光致电子转移过程。研究了pH值、BSA-AuNCs用量、温度和反应时间对氯霉素检测的影响。在最佳条件下，能够检测到痕量的CAP。线性工作范围为0.10 - 70.00 μM，检测限为33 nM（S/N = 3）。此外，所提出的方法已成功应用于牛奶样品中CAP的检测，并极大地改进了光谱法在CAP定量分析中的应用。
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