Mulec Janez, Skok Sara, Tomazin Rok, Letić Jasmina, Pliberšek Tadej, Stopinšek Sanja, Simčič Saša
Karst Research Institute, Research Centre of the Slovenian Academy of Sciences and Arts, Titov Trg 2, SI-6230 Postojna, Slovenia.
UNESCO Chair on Karst Education, University of Nova Gorica, Glavni Trg 8, SI-5271 Vipava, Slovenia.
Microorganisms. 2023 Mar 22;11(3):809. doi: 10.3390/microorganisms11030809.
A natural cave environment subject to regular human visitation was selected for aerobiological study to minimize the effects of severe temperature fluctuations, UV radiation, and desiccation stress on the aerobiome. The longer sampling period of bioaerosols, up to 22 months, was generally not associated with a proportionally incremental and cumulative increase of microbial biomass. The culture-independent biomass indicator ATP enabled quick and reliable determination of the total microbial biomass. Total airborne microbial biomass was influenced by human visitation to the cave, as confirmed by significantly higher concentrations being observed along tourist footpaths ( < 0.05). Airborne beta-glucans (BG) and lipopolysaccharide (LPS) are present in cave air, but their impact on the cave remains to be evaluated. spp., as an indicator of human presence, was detected at all sites studied. Their long-term survival decrease is likely due to high relative humidity, low temperature, the material to which they adhere, and potentially natural elevated radon concentration. The most commonly recorded species were: , which was identified in 52% of the studied sites, in 29%, and in 24% of the studied sites. Only a few isolates were assigned to Risk group 2: and .
为尽量减少剧烈温度波动、紫外线辐射和干燥胁迫对空气微生物群落的影响，选择了一个有人类定期探访的天然洞穴环境进行空气生物学研究。生物气溶胶长达22个月的较长采样期，通常与微生物生物量按比例增加和累积增加无关。与培养无关的生物量指标ATP能够快速可靠地测定总微生物生物量。洞穴中的人类探访会影响空气中的总微生物生物量，这一点已通过沿游客步道观察到的显著更高浓度得到证实（<0.05）。洞穴空气中存在空气传播的β-葡聚糖（BG）和脂多糖（LPS），但其对洞穴的影响仍有待评估。作为人类存在指标的 spp. 在所有研究地点均被检测到。它们长期生存能力下降可能是由于高相对湿度、低温、它们附着的材料以及潜在的天然氡浓度升高。最常记录的物种是：在所研究地点的52%中被鉴定出，在所研究地点的29%中被鉴定出，在所研究地点的24%中被鉴定出。只有少数分离株被归为风险组2： 和 。
