使用亲水相互作用液相色谱-质谱联用技术（HILIC-LC-MS），对实验室中从卵开始圈养繁殖的日本红腹蝾螈（Cynops pyrrhogaster）幼体中河豚毒素及其类似物的缺失进行确认。
Confirmation of the absence of tetrodotoxin and its analogues in the juveniles of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared from eggs in the laboratory using HILIC-LC-MS.
作者信息
Kudo Yuta, Chiba Chikafumi, Konoki Keiichi, Cho Yuko, Yotsu-Yamashita Mari
机构信息
Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan.
Faculty of Life and Environmental Sciences, University of Tsukuba, Tennoudai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan.
出版信息
Toxicon. 2015 Jul;101:101-5. doi: 10.1016/j.toxicon.2015.05.008. Epub 2015 May 16.
The tetrodotoxin (TTX) contents of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared from eggs to metamorphosed juveniles with a non-toxic diet for 70 weeks, as well as wild-caught juvenile newts, were investigated using a high-resolution hydrophilic interaction chromatography-LC-MS. TTX was detected in 0- to 22-week-old captive-reared juvenile newts but was not detected (<15 ng/g) in the 36- to 70-week-old newts, while significant levels of TTX (1.3-14 μg/g) were detected in the wild-caught juveniles.
利用高分辨率亲水相互作用色谱-液相色谱-质谱联用技术，对日本红腹蝾螈（Cynops pyrrhogaster）从卵饲养至变态幼体阶段（为期70周，采用无毒饮食）的圈养个体以及野生捕获的幼体蝾螈体内的河豚毒素（TTX）含量进行了研究。在0至22周龄的圈养幼体蝾螈中检测到了TTX，但在36至70周龄的蝾螈中未检测到（<15纳克/克），而在野生捕获的幼体中检测到了显著水平的TTX（1.3至14微克/克）。
Zotero 插件