Comparative Transcriptome Analysis of Flower Senescence of .

BACKGROUND

Flower senescence is the last stage of flower development and affects the ornamental and economic value of flower plants. There is still less known on flower senescence of the ornamental plant , a precious species of with significant commercial application value.

METHODS

Transcriptome sequencing was used to investigate the flower senescence in five developmental stages of .

RESULTS

By Illumina HiSeq sequencing, we generated approximately 101.16 Gb clean data and 46649 differentially expressed unigenes. Based on the different expression pattern, differentially expressed unigenes were classified into 10 Sub Class. And Sub Class 9 including 8252 unigenes, was highly expressed in the flower senescent stage, suggesting it had a potential regulatory relationship of flower senescence. First, we found that ethylene biosynthesis genes , , receptor genes and signaling genes , all upregulated during flower senescence, suggesting ethylene might play a key role in the flower senescence of . Furthermore, reactive oxygen species (ROS) production related genes (), (), (), and ROS scavenging related genes (), () and () were induced in senescent stage, suggesting ROS might be involved in the flower senescence. Besides, the expression of monoterpenoid and isoflavonoid biosynthesis genes, transcription factors (, , and ), also were increased during flower senescence.

CONCLUSION

In , ethylene pathway might be the key to regulate flower senescence, and ROS signal might play a role in the flower senescence.