Matsumoto I, Uchida K, Nakashima K, Hiyoshi S, Chambers J K, Tsujimoto H, Nakayama H
1 Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
2 Japan Small Animal Medical Center, Tokorozawa, Saitama, Japan.
Vet Pathol. 2018 Jan;55(1):98-107. doi: 10.1177/0300985817690212. Epub 2017 Feb 21.
Molecular clonality analysis of T-cell receptor (TCR) genes for diagnosing T-cell lymphoma is widely used in veterinary medicine. However, differentiating chronic enteritis (CE) from intestinal lymphoma is challenging because of the incompatibility between histopathologic and clonality analysis results. On the basis of findings that canine intestinal T-cell lymphoma and celiac disease share some common features, we conducted serologic examinations in combination with histopathologic and T-cell receptor clonality analyses in 48 dogs diagnosed with either CE or intestinal lymphoma. Immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies against gliadin and tissue transglutaminase (tTG) were quantitatively measured using ELISA. The conditions were classified according to the histopathologic diagnosis, clonality analysis, and combined histopathologic/clonality analysis. Histopathologic analysis showed that dogs with intestinal lymphoma were likely to have high levels of serum IgA antibodies against gliadin and tTG, and serum IgG antibodies against tTG. No correlation between the diagnosed groups and control group was observed in the results of the clonality analysis and histopathologic/clonality analysis. It is interesting that dogs with intestinal lymphoma had a higher serum IgA titer against gliadin and tTG than did dogs with CE. These results suggest an association between repetitive inflammatory stimulation by gliadin peptides and subsequent intestinal lymphoma in dogs.
用于诊断T细胞淋巴瘤的T细胞受体（TCR）基因的分子克隆性分析在兽医学中被广泛应用。然而，由于组织病理学和克隆性分析结果之间的不一致性，区分慢性肠炎（CE）和肠道淋巴瘤具有挑战性。基于犬肠道T细胞淋巴瘤与乳糜泻有一些共同特征的发现，我们对48只被诊断为CE或肠道淋巴瘤的犬进行了血清学检查，并结合组织病理学和T细胞受体克隆性分析。使用酶联免疫吸附测定法（ELISA）定量检测了抗麦醇溶蛋白和组织转谷氨酰胺酶（tTG）的免疫球蛋白A（IgA）和免疫球蛋白G（IgG）抗体。根据组织病理学诊断、克隆性分析以及组织病理学/克隆性联合分析对病情进行分类。组织病理学分析表明，患有肠道淋巴瘤的犬血清中抗麦醇溶蛋白和tTG的IgA抗体水平以及抗tTG的IgG抗体水平可能较高。在克隆性分析和组织病理学/克隆性分析结果中，未观察到诊断组与对照组之间存在相关性。有趣的是，患有肠道淋巴瘤的犬血清中抗麦醇溶蛋白和tTG的IgA滴度高于患有CE的犬。这些结果表明，麦醇溶蛋白肽的反复炎症刺激与犬随后发生的肠道淋巴瘤之间存在关联。
