Martínez-Cortés G, Gusmão L, Pereira R, Salcido V H, Favela-Mendoza A F, Muñoz-Valle J F, Inclán-Sánchez A, López-Hernández L B, Rangel-Villalobos H
Instituto de Investigación en Genética Molecular, Universidad de Guadalajara (CUCiénega-UdeG), Ocotlán, Jalisco, Mexico.
IPATIMUP Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal; DNA Diagnostic Laboratory (LDD), State University of Rio de Janeiro (UERJ), Rio de Janeiro, Brazil.
Forensic Sci Int Genet. 2015 Jul;17:149-152. doi: 10.1016/j.fsigen.2015.04.011. Epub 2015 May 2.
Insertion-deletions for human identification purposes (HID-Indels) offer advantages to solve particular forensic situations and complex paternity cases. In Mexico, admixed population known as Mestizos is the largest (∼90%), plus a number of Amerindian groups (∼10%), which have not been studied with HID-Indels. For this reason, allele frequencies and forensic parameters for 38 HID-Indels were estimated in 531 unrelated individuals from one Amerindian (Purépecha) and seven Mestizo populations from different regions of the country. Genotype distribution was in agreement with Hardy-Weinberg expectations in almost all loci/populations. The linkage disequilibrium (LD) test did not reveal possible associations between loci pairs in all eight Mexican populations. The combined power of discrimination was high in all populations (PD >99.99999999998%). However, the power of exclusion of the 38 HID-Indel system (PE >99.6863%) was reduced regarding most of autosomal STR kits. The assessment of genetic structure (AMOVA) and relationships between populations (FST) demonstrated significant differences among Mexican populations, mainly of the Purépecha Amerindian group. Among Mexican-Mestizos, three population clusters consistent with geography were defined: (i) North-West region: Chihuahua, Sinaloa, and Jalisco; (ii) Central-Southern region: Mexico City, Veracruz and Yucatan; (iii) South region: Chiapas. In brief, this report validates the inclusion of the 38 HID-Indel system in forensic casework and paternity cases in seven Mexican-Mestizo populations from different regions, and in one Mexican Amerindian group.
用于人类身份识别的插入缺失（HID-Indels）在解决特定法医案件和复杂亲子鉴定案件方面具有优势。在墨西哥，被称为梅斯蒂索人的混血人群数量最多（约90%），此外还有一些美洲印第安人群体（约10%），尚未对这些群体进行HID-Indels研究。因此，在来自一个美洲印第安群体（普雷佩查）以及该国不同地区的七个梅斯蒂索人群体的531名无关个体中，估计了38个HID-Indels的等位基因频率和法医参数。几乎在所有位点/群体中，基因型分布均符合哈迪-温伯格预期。连锁不平衡（LD）检验未揭示所有八个墨西哥群体中位点对之间可能存在的关联。所有群体的联合鉴别力均较高（PD>99.99999999998%）。然而，与大多数常染色体STR试剂盒相比，38个HID-Indel系统的排除力（PE>99.6863%）有所降低。遗传结构评估（AMOVA）和群体间关系（FST）表明，墨西哥群体之间存在显著差异，主要是普雷佩查美洲印第安群体。在墨西哥梅斯蒂索人群体中，定义了与地理区域一致的三个群体聚类：（i）西北地区：奇瓦瓦州、锡那罗亚州和哈利斯科州；（ii）中南部地区：墨西哥城、韦拉克鲁斯州和尤卡坦州；（iii）南部地区：恰帕斯州。简而言之，本报告验证了38个HID-Indel系统可用于来自不同地区的七个墨西哥梅斯蒂索人群体以及一个墨西哥美洲印第安群体的法医案件和亲子鉴定案件。
