Martínez-Cortés G, Zuñiga-Chiquette F, Celorio-Sánchez A S, Ruiz García E, Antelo-Figueroa A B, Dalpozzo-Valenzuela V, Valenzuela-Coronado A, Rangel-Villalobos H
Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCiénega-UdeG), Av. Universidad #1115, Col. Paso Blanco, CP 47810, Ocotlán, Jalisco, Mexico.
Laboratorio de Genética Forense, Servicios Periciales de la Procuraduría de Justicia del Estado de Baja California, Tijuana, Baja California, Mexico.
Int J Legal Med. 2019 May;133(3):781-783. doi: 10.1007/s00414-018-1950-1. Epub 2018 Oct 17.
Mexican Mestizos (admixed) have been poorly studied for short tandem repeats (STRs) included for new human identification (HID) kits, such as the GlobalFiler PCR Amplification kit. Therefore, this kit was analyzed in 784 unrelated volunteers from the city of Tijuana (n = 381) and Sonora state (n = 403) in the northwest region of Mexico. Allele frequencies, forensic parameters, Hardy-Weinberg equilibrium, and linkage equilibrium were estimated or evaluated for 21 autosomal STRs, respectively. For this HID kit, the combined power of discrimination (PD) was > 0.99999999999999 (RMP range = 1.23 to 3.0 × 10), and the combined power of exclusion (PE) were 0.999999993 and 0.999999997 in Tijuana city and Sonora state, respectively. Interpopulation analyses based on STRs of the GlobalFiler kit was performed, including four Mexican Native American, one Mexican Mestizo, and four ethnic American populations (USA), previously studied. The low-but significant-differentiation observed among Mexican Mestizos (F = 0.0969%; p = 0.02584) justifies the creation of STR databases for HID purposes in this country. In brief, results allow the confident use of the GlobalFiler kit for HID purposes in Mestizo population from the Northwest region Mexico.
对于新的人类身份识别(HID)试剂盒中包含的短串联重复序列(STR),墨西哥混血儿(混血人种)的相关研究较少,比如GlobalFiler PCR扩增试剂盒。因此,对来自墨西哥西北部地区蒂华纳市(n = 381)和索诺拉州(n = 403)的784名无亲缘关系的志愿者进行了该试剂盒分析。分别估计或评估了21个常染色体STR的等位基因频率、法医参数、哈迪-温伯格平衡和连锁平衡。对于这个HID试剂盒,在蒂华纳市和索诺拉州,其联合鉴别力(PD)均> 0.99999999999999(随机匹配概率范围 = 1.23至3.0×10),联合排除力(PE)分别为0.999999993和0.999999997。基于GlobalFiler试剂盒的STR进行了群体间分析,包括之前研究过的四个墨西哥原住民、一个墨西哥混血儿和四个美国族裔群体。在墨西哥混血儿中观察到的低但显著的分化(F = 0.0969%;p = 0.02584)证明了在该国建立用于HID目的的STR数据库的合理性。简而言之,研究结果使得GlobalFiler试剂盒能够可靠地用于墨西哥西北部地区混血人群的HID目的。