Chakraborty Samrat, Ghosh Mandakini, Chakraborti Srijita, Jana Sougata, Sen Kalyan Kumar, Kokare Chandrakant, Zhang Lixin
Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.
Department of Pharmaceutics, Gupta College of Technological Sciences, Asansol 713301, WB, India.
Int J Biol Macromol. 2015 Aug;79:405-12. doi: 10.1016/j.ijbiomac.2015.04.068. Epub 2015 May 16.
This investigation aims to isolate an Actinomycetes strain producing a biosurfactant from the unexplored region of industrial and coal mine areas. Actinomycetes are selected for this study as their novel chemistry was not exhausted and they have tremendous potential to produce bioactive secondary metabolites. The biosurfactant was characterized and further needed to be utilized for pharmaceutical dosage form. Isolation, purification, screening, and characterization of the Actinomycetes A17 were done followed by its fermentation in optimized conditions. The cell-free supernatant was used for the extraction of the biosurfactant and precipitated by cold acetone. The dried precipitate was purified by TLC and the emulsification index, surface tension and CMC were determined. The isolated strain with preferred results was identified as Actinomycetes nocardiopsis A17 with high foam-forming properties. It gives lipase, amylase, gelatinase, and protease activity. The emulsification index was found to be 93±0.8 with surface tension 66.67 dyne/cm at the lowest concentration and cmc 0.6 μg/ml. These biosurfactants were characterized by Fourier transform infra red (FT-IR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS). Therefore, it can be concluded that the biosurfactant produced by Actinomycetes nocardiopsis sp. strain A17 was found to have satisfactory results with high surface activity and emulsion-forming ability.
本研究旨在从工业和煤矿区未开发区域分离出一株能产生生物表面活性剂的放线菌菌株。选择放线菌进行本研究，是因为其新颖的化学性质尚未被穷尽，且它们具有产生生物活性次级代谢产物的巨大潜力。对该生物表面活性剂进行了表征，并且进一步需要将其用于药物剂型。对放线菌A17进行了分离、纯化、筛选和表征，随后在优化条件下进行发酵。无细胞上清液用于提取生物表面活性剂，并用冷丙酮沉淀。干燥后的沉淀物通过薄层层析进行纯化，并测定乳化指数、表面张力和临界胶束浓度。分离出的具有较好结果的菌株被鉴定为具有高泡沫形成特性的诺卡氏放线菌A17。它具有脂肪酶、淀粉酶、明胶酶和蛋白酶活性。乳化指数为93±0.8，最低浓度下的表面张力为66.67达因/厘米，临界胶束浓度为0.6微克/毫升。这些生物表面活性剂通过傅里叶变换红外光谱（FT-IR）和液相色谱-质谱联用（LC-MS）进行表征。因此，可以得出结论，诺卡氏放线菌属菌株A17产生的生物表面活性剂具有令人满意的结果，具有高表面活性和乳化形成能力。
