Roszak Dorota, Gałęcka Mirosława, Cichy Wojciech, Szachta Patrycja
Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poland.
Institute of Microecology, Poznan, Poland.
Adv Med Sci. 2015 Sep;60(2):246-52. doi: 10.1016/j.advms.2015.04.003. Epub 2015 Apr 29.
The optimization of procedure evaluating the severity of inflammatory bowel diseases (IBD) using non-invasive methods.
PATIENTS/METHODS: One hundred and nine children with IBD hospitalized in gastroenterology ward between 2009 and 2011 participated in the study. Activity of the disease was evaluated in each patient. Concentration of three inflammatory markers: dimeric form of tumor pyruvate kinase (M2-PK), calprotectin and lactoferrin was evaluated using immunoenzymatic tests.
Existence of a significant correlation between the faecal level of all tested markers and the stage of clinical activity of the disease was demonstrated in children with IBD, both in Crohn's disease (M2-PK p<0.01; calprotectin p=0.005; lactoferrin p<0.01) and in ulcerative colitis group (M2-PK p<0.01; calprotectin p=0.004; lactoferrin p<0.01). A significant difference in the level of markers was found between children with unclassified colitis and the group of patients with ulcerative colitis and Crohn's disease, but there was no difference between Crohn's disease and ulcerative colitis. The increase in the level of one marker correlated with increasing level of other markers (p<0.01). Faecal markers seem to correlate well with majority of indicators of inflammatory condition in blood.
Measuring M2-PK, lactoferrin and calprotectin levels in faeces seem to be a useful indicator of the level of disease activity in children with IBD.
优化使用非侵入性方法评估炎症性肠病（IBD）严重程度的程序。
患者/方法：2009年至2011年间在胃肠病科病房住院的109例IBD患儿参与了该研究。对每位患者的疾病活动度进行评估。使用免疫酶试验评估三种炎症标志物的浓度：肿瘤丙酮酸激酶二聚体形式（M2-PK）、钙卫蛋白和乳铁蛋白。
在IBD患儿中，无论是克罗恩病（M2-PK p<0.01；钙卫蛋白p=0.005；乳铁蛋白p<0.01）还是溃疡性结肠炎组（M2-PK p<0.01；钙卫蛋白p=0.004；乳铁蛋白p<0.01），均证实所有检测标志物的粪便水平与疾病临床活动阶段之间存在显著相关性。未分类结肠炎患儿与溃疡性结肠炎和克罗恩病患者组之间的标志物水平存在显著差异，但克罗恩病和溃疡性结肠炎之间无差异。一种标志物水平的升高与其他标志物水平的升高相关（p<0.01）。粪便标志物似乎与血液中大多数炎症指标密切相关。
检测粪便中M2-PK、乳铁蛋白和钙卫蛋白水平似乎是IBD患儿疾病活动水平的有用指标。
