Kretzschmar R, Ermisch A
Dept. of Cell Biology and Regulation, Karl-Marx-University, Leipzig, GDR.
Exp Clin Endocrinol. 1989 Sep;94(1-2):151-6. doi: 10.1055/s-0029-1210891.
The binding of [125I]arginine-8-vasopressin (AVP) to hippocampal microvessels isolated from brains of normal Wistar rats, animals after water deprivation and heterozygous as well as homozygous diabetes-insipidus rats (Brattleboro strain) were measured. Data from binding experiments from the microvessels of the different groups of animals in each case revealed a single class of high affinity binding sites. However, the binding parameters between the different groups of rats were different. The affinity constants differs by a factor of 5.1, ranging from KD = 1.18 nmol X L-1 (animals after water deprivation) to KD = 6.05 nmol X L-1 (homozygous Brattleboro rats). The binding capacity, differing by a factor of 3.5, ranged from Bmax = 245 fmol X mg-1 to Bmax = 865 fmol X mg-1.
测定了[125I]精氨酸-8-加压素（AVP）与从正常Wistar大鼠、禁水后的动物以及杂合子和纯合子尿崩症大鼠（Brattleboro品系）大脑中分离出的海马微血管的结合情况。每组动物微血管结合实验的数据在每种情况下均显示出一类高亲和力结合位点。然而，不同组大鼠之间的结合参数有所不同。亲和常数相差5.1倍，范围从KD = 1.18 nmol×L-1（禁水后的动物）到KD = 6.05 nmol×L-1（纯合子Brattleboro大鼠）。结合容量相差3.5倍，范围从Bmax = 245 fmol×mg-1到Bmax = 865 fmol×mg-1。
