Kretzschmar R, Landgraf R, Gjedde A, Ermisch A
Brain Res. 1986 Aug 20;380(2):325-30. doi: 10.1016/0006-8993(86)90229-5.
Recent evidence suggests that vasopressin may influence the permeability of the endothelium of brain capillaries. We measured the binding of [125I]arginine-8-vasopressin ([125I]AVP) to microvessels isolated from different regions of the rat brain. The study revealed saturable and specific binding of [125I]AVP to microvessels isolated from hippocampus. Scatchard analysis confirmed a single class of high affinity sites with an equilibrium dissociation constant, Kd, of 3.2 nM and an apparent maximal binding capacity of 205 fmol/mg protein. No binding was observed to microvessels from neocortex and striatum.
最近的证据表明，血管加压素可能会影响脑毛细血管内皮的通透性。我们测量了[125I]精氨酸-8-血管加压素（[125I]AVP）与从大鼠脑不同区域分离出的微血管的结合情况。研究发现，[125I]AVP与从海马体分离出的微血管存在可饱和的特异性结合。Scatchard分析证实存在一类单一的高亲和力位点，其平衡解离常数Kd为3.2 nM，表观最大结合容量为205 fmol/mg蛋白质。未观察到[125I]AVP与新皮层和纹状体的微血管有结合。
