Pearlmutter A F, Szkrybalo M, Kim Y, Harik S I
Department of Biochemistry, Medical College of Ohio, Toledo 43699.
Neurosci Lett. 1988 Apr 22;87(1-2):121-6. doi: 10.1016/0304-3940(88)90156-5.
We tested for the presence of arginine vasopressin (AVP) receptors in pig cerebral microvessels, cerebral cortex and hippocampus by specific binding methods with [3H]AVP as the ligand. The specific binding of [3H]AVP to all preparations was saturable and Scatchard analysis indicated a single class of high affinity binding sites (dissociation constant of 1-2 nM). Maximal binding capacity in cerebral microvessels was about 60% that of the cerebral cortex; and there were no apparent differences in the maximal binding capacity between cerebral cortex and hippocampus. These findings suggest the existence of AVP receptor sites in cerebral microvessels and support the hypothesis that AVP has a role in the control of the brain microcirculation.
我们采用以[3H]精氨酸加压素（AVP）为配体的特异性结合方法，检测猪脑微血管、大脑皮层和海马体中AVP受体的存在情况。[3H]AVP与所有制剂的特异性结合具有饱和性，Scatchard分析表明存在一类单一的高亲和力结合位点（解离常数为1 - 2 nM）。脑微血管中的最大结合容量约为大脑皮层的60%；大脑皮层和海马体之间的最大结合容量没有明显差异。这些发现提示脑微血管中存在AVP受体位点，并支持AVP在脑微循环控制中起作用这一假说。
