Lankin Vadim Z, Konovalova Galina G, Tikhaze Alla K, Shumaev Konstantin B, Belova Kumskova Elena M, Grechnikova Maria A, Viigimaa Margus
Russian Cardiology Research Complex, Moscow, Russia.
Tallinn University of Technology, Tallinn, Estonia.
J Diabetes. 2016 May;8(3):398-404. doi: 10.1111/1753-0407.12309. Epub 2015 Sep 30.
The aim of the present study was to examine the effect of aldehyde modification on antioxidant enzyme activity in diabetic patients.
The activity of commercially available antioxidant enzymes (catalase, glutathione peroxidase [GPx], and Cu,Zn-superoxide dismutase [SOD]) was determined in vitro prior to and after aldehyde modification. The activity of erythrocyte Cu,Zn-SOD was assayed in blood drawn from healthy donors, diabetic patients with decompensated carbohydrate metabolism, and diabetic patients after glucose-lowering therapy.
In vitro aldehyde modification had no effect on catalase activity, but diminished GPx and Cu,Zn-SOD activity. In diabetic patients with decompensated carbohydrate metabolism, glucose-lowering therapy significantly increased Cu,Zn-SOD activity, the effect being especially pronounced after administration of metformin.
It is likely that metformin antagonizes the aldehyde-induced inhibition of erythrocyte Cu,Zn-SOD in diabetic patients more effectively than sulfonylurea drugs.
本研究的目的是检测醛修饰对糖尿病患者抗氧化酶活性的影响。
在醛修饰前后，体外测定市售抗氧化酶（过氧化氢酶、谷胱甘肽过氧化物酶[GPx]和铜锌超氧化物歧化酶[SOD]）的活性。从健康供体、碳水化合物代谢失代偿的糖尿病患者以及降糖治疗后的糖尿病患者抽取的血液中检测红细胞铜锌超氧化物歧化酶的活性。
体外醛修饰对过氧化氢酶活性无影响，但降低了GPx和铜锌超氧化物歧化酶的活性。在碳水化合物代谢失代偿的糖尿病患者中，降糖治疗显著提高了铜锌超氧化物歧化酶的活性，二甲双胍给药后效果尤为明显。
二甲双胍可能比磺脲类药物更有效地拮抗醛诱导的糖尿病患者红细胞铜锌超氧化物歧化酶的抑制作用。
