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在角芽区域注射丁香油精华后对去芽山羊羔的研究。

Study of disbudding goat kids following injection of clove oil essence in horn bud region.

作者信息

Molaei Mohammad Mahdi, Mostafavi Ali, Kheirandish Reza, Azari Omid, Shaddel Mohsen

机构信息

Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;

Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran;

出版信息

Vet Res Forum. 2015 Winter;6(1):17-22. Epub 2015 Mar 15.

Abstract

This study was performed to evaluate the efficacy of injection of essential oil of Eugenia caryophyllata in the kid horn buds, as a new chemical technique for disbudding. Five-day-old healthy goat kids from both sexes (n = 16) were divided randomly into 4 equal groups. In groups 1, 2 and 3, 0.2 mL of clove essence and in group 4 (control) 0.2 mL of normal saline was injected into the left horn bud of goat kids. Right horn bud in all kids was considered to ensure that they are horned. During the study, the rate of horn growth were evaluated in determined time intervals between groups 1 and 4. Tissue samples were taken from right and left horn bud in groups 2 and 3, at five and ten days after clove essence injection, for microscopic study. The results of the study showed that the clove essence stopped horn growth, whereas there was no significant difference in horn growth rate between left and right horns after injection of normal saline, in group 4. Histopathological study showed that injection of clove essence caused complete necrosis of epidermis and underlying dermis with collagenolysis in horn bud tissues, 5 days after injection and then progress in healing process was observed after 10 days. According to the results of this study, it can be concluded that the injection of clove essence is an effective method to stop horn growth without any undesirable effects on clinical parameters in goat kids.

摘要

本研究旨在评估注射丁香精油于羔羊角芽中的效果,作为一种新的去角化学技术。选取16只5日龄的健康雌雄羔羊,随机分为4组,每组数量相等。第1、2和3组,向羔羊的左角芽注射0.2 mL丁香精油,第4组(对照组)向羔羊的左角芽注射0.2 mL生理盐水。所有羔羊的右角芽均保留以确保其有角。在研究过程中,对第1组和第4组在特定时间间隔内的角生长速率进行评估。在第2组和第3组注射丁香精油后的第5天和第10天,从左右角芽采集组织样本进行显微镜检查。研究结果表明,丁香精油可抑制角生长,而第4组注射生理盐水后,左右角的生长速率无显著差异。组织病理学研究表明,注射丁香精油5天后,角芽组织中的表皮和下方真皮出现完全坏死并伴有胶原溶解,10天后观察到愈合过程进展。根据本研究结果,可以得出结论,注射丁香精油是一种有效的抑制羔羊角生长的方法,且对羔羊的临床参数无任何不良影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e15/4405681/b8d64caaed05/vrf-6-017-g001.jpg

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